Katnik I, Steuden I, Pupek M, Wiedłocha A, Dobryszycka W
Department of Pharm. Biochemistry, Medical Academy, Wrocław, Poland.
Hybridoma. 1989 Oct;8(5):551-60. doi: 10.1089/hyb.1989.8.551.
Three monoclonal antibodies: 2.36.71.41, 7.60.66.55, and 18.4.40. 80 to human haptoglobin 2-1 were produced, purified and characterized. The affinity constants ranged within 0.3-2.4 x 10(8) M-1. The monoclonal antibodies 7.60.66.55 and 18.4.40.80 reacted with beta subunit of haptoglobin, showed similar epitope affinities and epitope densities on main haptoglobin types. However, the epitope on the haptoglobin molecule for the monoclonal antibody 18.4.40.80 occupied somewhat more surface than that for the antibody 7.60.66.55. The monoclonal antibody 2.36.71.41 was able to bind both alpha and beta chains of haptoglobin. In ELISA affinity reactions this antibody achieved with haptoglobin 2-2 the plateau phase at absorbance values 15% higher than with haptoglobin 2-1, and 60% higher than with haptoglobin 1-1. End-point titration of the monoclonal antibody 2.36.71.41 against three haptoglobin types showed differences in titer, indicating distinct epitope densities.
制备、纯化并鉴定了三种针对人触珠蛋白2-1的单克隆抗体：2.36.71.41、7.60.66.55和18.4.40.80。亲和常数范围为0.3 - 2.4×10⁸ M⁻¹。单克隆抗体7.60.66.55和18.4.40.80与触珠蛋白的β亚基反应，在主要触珠蛋白类型上显示出相似的表位亲和力和表位密度。然而，单克隆抗体18.4.40.80在触珠蛋白分子上的表位比抗体7.60.66.55占据的表面稍多。单克隆抗体2.36.71.41能够结合触珠蛋白的α链和β链。在ELISA亲和反应中，该抗体与触珠蛋白2-2达到平台期时的吸光度值比与触珠蛋白2-1高15%，比与触珠蛋白1-1高60%。单克隆抗体2.36.71.41针对三种触珠蛋白类型的终点滴定显示出滴度差异，表明表位密度不同。
