一种抗牛触珠蛋白单克隆抗体的制备、鉴定及其在酶联免疫吸附测定中的应用
Production and characterization of a monoclonal antibody against bovine haptoglobin and its use in an ELISA.
作者信息
Sheffield C L, Kamps-Holtzapple C, DeLoach J R, Stanker L H
机构信息
US Department of Agriculture, Agricultural Research Service, College Station, TX 77845.
出版信息
Vet Immunol Immunopathol. 1994 Aug;42(2):171-83. doi: 10.1016/0165-2427(94)90006-x.
Haptoglobin (Hp) is the major acute phase reactant found in cattle. As such, it is an excellent indicator of early disease processes and could be used as a marker for pre-clinical illness in cattle. The production of monoclonal antibodies (mAbs) directed against bovine Hp and bovine hemoglobin is described. The anti-haptoglobin mAbs (Hap1, Hap2, Hap3) and the anti-bovine hemoglobin (Hb) mAb (BoHem1) were characterized and tested for cross-reactivity by means of enzyme-linked immunosorbent assay (ELISA) and immunoblotting analyses. Additionally, the development of an ELISA based on an anti-haptoglobin mAb is discussed.
触珠蛋白（Hp）是牛体内发现的主要急性期反应物。因此，它是早期疾病进程的极佳指标，可作为牛临床前疾病的标志物。本文描述了针对牛Hp和牛血红蛋白的单克隆抗体（mAb）的制备。通过酶联免疫吸附测定（ELISA）和免疫印迹分析对抗触珠蛋白单克隆抗体（Hap1、Hap2、Hap3）和抗牛血红蛋白（Hb）单克隆抗体（BoHem1）进行了表征和交叉反应性测试。此外，还讨论了基于抗触珠蛋白单克隆抗体的ELISA的开发。
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