Baker Nicholas E, Li Ke, Quiquand Manon, Ruggiero Robert, Wang Lan-Hsin
Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States; Department of Ophthalmology and Visual Sciences, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States; Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States.
Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States.
Methods. 2014 Jun 15;68(1):252-9. doi: 10.1016/j.ymeth.2014.04.007. Epub 2014 Apr 29.
The eye has been one of the most intensively studied organs in Drosophila. The wealth of knowledge about its development, as well as the reagents that have been developed, and the fact that the eye is dispensable for survival, also make the eye suitable for genetic interaction studies and genetic screens. This article provides a brief overview of the methods developed to image and probe eye development at multiple developmental stages, including live imaging, immunostaining of fixed tissues, in situ hybridizations, and scanning electron microscopy and color photography of adult eyes. Also summarized are genetic approaches that can be performed in the eye, including mosaic analysis and conditional mutation, gene misexpression and knockdown, and forward genetic and modifier screens.
眼睛一直是果蝇中研究最为深入的器官之一。关于其发育的丰富知识，以及已开发出的试剂，再加上眼睛对于生存并非必需这一事实，也使得眼睛适用于基因相互作用研究和基因筛选。本文简要概述了为在多个发育阶段对眼睛发育进行成像和探测而开发的方法，包括活体成像、固定组织的免疫染色、原位杂交，以及成体眼睛的扫描电子显微镜检查和彩色摄影。还总结了可在眼睛中进行的遗传方法，包括镶嵌分析和条件突变、基因错误表达和敲低，以及正向遗传学和修饰因子筛选。
