Siqueira L F, de Almeida R G, Francisco W, dos Santos Júnior M F, Belda W
Departamento de Epidemiologia da Faculdade de Saúde Pública da Universidade de São Paulo.
Hansenol Int. 1987 Jun;12(1):8-11.
The fuchsin salts currently available in Latin American market have shown some instability when in solution, according to the classic Ziehl-Neelsen method, resulting in a total precipitation of the salt. The authors indicate a new technique for the preparation of this solution, in order to minimize the action of interfering factors responsible for the precipitation, obtaining thus a greater solubility of the salt, as well as the solution stability. The method effectiveness is reinforced by the utilization of a smaller amount of the salt and the attainment of a larger storage period for the solution.