Holm I E
Department of Neurobiology, University of Aarhus, Denmark.
Histochemistry. 1989;92(4):301-6. doi: 10.1007/BF00500544.
Two histochemical methods for visualization of zinc in synaptic vesicles, the Neo-Timm and selenium methods, have been shown to additionally stain glial cells and neuronal somata. In a previous light microscopic study the majority of stained glial cells were seen in the major fiber tracts of the rat telencephalon. The aim of the present study was to further characterize the stained glial cells with respect to glial cell type and ultrastructural localization of the silver grains responsible for the staining. Electron microscopic analysis of brains treated according to either method revealed that the vast majority of stained glial cells belonged to the dark oligodendroglial cell type. However, a smaller number of stained astrocytes was also seen, especially in the grey matter. The silver grains responsible for the staining were located in electron-dense rounded cytoplasmic organelles, suggestive of lysosomes.
两种用于显示突触小泡中锌的组织化学方法，即新蒂姆法和硒法，已被证明还会对神经胶质细胞和神经元胞体进行染色。在先前的一项光学显微镜研究中，大多数被染色的神经胶质细胞见于大鼠端脑的主要纤维束中。本研究的目的是根据神经胶质细胞类型以及负责染色的银颗粒的超微结构定位，进一步对被染色的神经胶质细胞进行特征描述。对按照这两种方法处理的大脑进行电子显微镜分析显示，绝大多数被染色的神经胶质细胞属于暗型少突胶质细胞类型。然而，也可见到少量被染色的星形胶质细胞，尤其是在灰质中。负责染色的银颗粒位于电子致密的圆形细胞质细胞器中，提示为溶酶体。
