Holm I E, Andreasen A, Danscher G, Pérez-Clausell J, Nielsen H
Institute of Anatomy B, University of Aarhus, Denmark.
Histochemistry. 1988;89(3):289-93. doi: 10.1007/BF00493154.
By means of the Neo-Timm method it has recently been shown that zinc is present in a fraction of the round clear synaptic vesicles of certain boutons located primarily in telencephalic structures (Pérez-Clausell and Danscher 1985). It is believed that this zinc belongs to a fraction of the total brain zinc which is histochemically active (Frederickson and Danscher 1988) in that it can be visualized by means of e.g. the Neo-Timm and selenium methods (autometallography). The present study is based on the suggestion that the autometallographically developed zinc patterns represent a histochemical quantitative expression of this fraction of the total brain zinc. The different colours of the zinc pattern reflect local variations in the concentration of zinc containing vesicles. Large boutons with a high content of stained vesicles will show up darkly because of fusion of adjoining silver grains while smaller boutons with fewer zinc containing vesicles give rise to yellow staining of various shades. We have exploited this difference in staining of pattern by applying computerized optic densitometry to light microscopic sections treated according to the Neo-Timm and the selenium methods, respectively.
最近通过新蒂姆方法已表明，锌存在于主要位于端脑结构中的某些终扣的一部分圆形清亮突触小泡中（佩雷斯 - 克劳塞尔和丹舍尔，1985年）。据信，这种锌属于全脑锌的一部分，具有组织化学活性（弗雷德里克森和丹舍尔，1988年），因为它可以通过例如新蒂姆方法和硒方法（自动金属显影术）可视化。本研究基于这样的建议，即自动金属显影术显示的锌模式代表了全脑锌这一部分的组织化学定量表达。锌模式的不同颜色反映了含锌小泡浓度的局部差异。含有大量染色小泡的大终扣由于相邻银颗粒的融合而会显示为深色，而含锌小泡较少的较小终扣则会产生不同深浅的黄色染色。我们通过分别对根据新蒂姆方法和硒方法处理的光学显微镜切片应用计算机化光学密度测定法，利用了这种模式染色的差异。
