Rohani Fatemeh Ganjeizadeh, Aminaee Mohammad Mehdi, Kianfar Maryam
a Department of Plant Protection of Kerman's Agricultural and Natural Resources Research Center , Kerman 7617913739 , Iran.
Food Addit Contam Part B Surveill. 2011;4(3):191-4. doi: 10.1080/19393210.2011.599866. Epub 2011 Jul 21.
The aim of this study was to assess levels of aflatoxin M1 (AFM1) in milk samples from Kerman, Iran. AFM1 was detected in 72 samples, ranging in concentration from <0.01 to 0.41 µg l(-1). The samples were analyzed using immunoaffinity column for clean-up and HPLC for determining AFM1. Milk samples were collected from six dairy farms. AFM1 was found in ∼ 50% of the milk samples. The average level of AFM1 was below the tolerance limit (0.05 µg l(-1)), but 50% of the samples had greater levels than the maximum tolerance limit accepted by EU and the Iranian national standard. The method detection limit and limit of quantification were 0.01 and 0.03 µg l(-1), respectively, and recovery of the method was 87%. The results showed that AFM1 contamination is a serious problem for public health. To achieve a low level of AFM1 in milk, cattle feed must be monitored regularly for aflatoxin contamination and protected from fungal contamination as much as possible.
本研究的目的是评估伊朗克尔曼牛奶样本中的黄曲霉毒素M1（AFM1）水平。在72个样本中检测到了AFM1，其浓度范围为<0.01至0.41微克/升。使用免疫亲和柱进行净化，并用高效液相色谱法测定AFM1来分析样本。牛奶样本取自六个奶牛场。约50%的牛奶样本中发现了AFM1。AFM1的平均水平低于耐受限度（0.05微克/升），但50%的样本含量高于欧盟和伊朗国家标准所接受的最大耐受限度。该方法的检测限和定量限分别为0.01和0.03微克/升，方法回收率为87%。结果表明，AFM1污染对公众健康是一个严重问题。为了使牛奶中的AFM1含量较低，必须定期监测牛饲料中的黄曲霉毒素污染，并尽可能防止其受到真菌污染。
