Microbiology and Cell Science Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.
Plant Pathology Department, Citrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, FL, USA.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2461-2466. doi: 10.1099/ijs.0.063255-0. Epub 2014 Apr 30.
The Gram-stain-negative, rod-shaped bacterial isolate BT-1(T) is the closest relative to the genus 'Candidatus Liberibacter' cultured to date. BT-1(T) was recovered from the phloem sap of a defoliating mountain papaya in Puerto Rico. The BT-1(T) 16S rRNA gene sequence showed that strain BT-1(T) is most closely related to members of the genus 'Ca. Liberibacter' sharing 94.7% 16S rRNA gene sequence similarity with 'Ca. Liberibacter americanus' and 'Ca. Liberibacter asiaticus'. Additionally, average nucleotide identity, 16S rRNA gene sequences and conserved protein sequences supported inclusion of the previously described species of the genus 'Ca. Liberibacter' in a genus with BT-1(T). The prominent fatty acids of isolate BT-1(T) were C18 : 1ω7c (77.2%), C16 : 0 OH (4.8%), C18 : 0 (4.4%) and C16 : 0 (3.5%). Both physiological and genomic characteristics support the creation of the genus Liberibacter, as well as the novel species Liberibacter crescens gen. nov., sp. nov. with type strain BT-1(T) ( = ATCC BAA-2481(T) = DSM 26877(T)).
革兰氏阴性、杆状的细菌分离株 BT-1(T) 是迄今为止培养的与属“Candidatus Liberibacter”最接近的亲缘关系。BT-1(T) 从波多黎各的一种落叶山地木瓜的韧皮部汁液中回收。BT-1(T) 的 16S rRNA 基因序列表明，该菌株与属“Ca. Liberibacter”的成员最为密切相关，与“Ca. Liberibacter americanus”和“Ca. Liberibacter asiaticus”共享 94.7%的 16S rRNA 基因序列相似性。此外，平均核苷酸同一性、16S rRNA 基因序列和保守蛋白序列支持将先前描述的属“Ca. Liberibacter”的物种归入与 BT-1(T) 相同的属。分离株 BT-1(T) 的主要脂肪酸为 C18:1ω7c（77.2%）、C16:0 OH（4.8%）、C18:0（4.4%）和 C16:0（3.5%）。生理和基因组特征都支持创建 Liberibacter 属，以及新型 Liberibacter crescens gen. nov.，sp. nov.，其模式株为 BT-1(T)（ = ATCC BAA-2481(T) = DSM 26877(T)）。
