Mu Yang, Jia Wei-Bin, Ke Zhuang, Zhuang Wen, Wang Hong-Mei, Jiang Jian-Dong, Chen Kai, Chen Qing
1Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
2College of City and Architecture Engineering, Zaozhuang University, Zaozhuang, Shandong 277160, PR China.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2602-2606. doi: 10.1099/ijsem.0.002883. Epub 2018 Jun 27.
Strain AH-1, a Gram-negative, aerobic, non-spore-forming, motile, rod-shaped bacterium, was isolated from tetrabromobisphenol A-contaminated soil in China. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AH-1 was a member of the genus Shinella and showed the highest sequence similarity to Shinella fusca DC-196 (97.7 %), Shinella granuli Ch06 (97.3 %), Shinella daejeonensis MJ02 (97.1 %) and Shinella yambaruensis MS4 (96.8 %), and lower (<96.7 %) sequence similarity to other known Shinella species. Chemotaxonomic analysis revealed that strain AH-1 possessed Q-10 as the major isoprenoid quinone; and summed feature 8 (C18 : 1ω6c/C18 : 1ω7c), C16 : 0, C12 : 0 aldehyde, C18 : 0, C19 : 0 cyclo ω8c and C18 : 0 3-OH were the predominant fatty acids. Strain AH-1 showed low DNA-DNA relatedness to S. fusca DC-196 (28.6±5.7 %), S. granuli Ch06 (43.7±3.8 %) and S. daejeonensis MJ02 (48.1±2.6 %). The DNA G+C content was 68.2 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA-DNA hybridization, strain AH-1 is considered a novel species of the genus Shinella, for which the name Shinella pollutisoli sp. nov. (type strain AH-1=KCTC 52677=CCTCC AB 2017242) is proposed.
菌株AH-1是一种革兰氏阴性、需氧、无芽孢形成、具运动性的杆状细菌，从中国受四溴双酚A污染的土壤中分离得到。采用多相分类法对其分类地位进行了研究。基于16S rRNA基因序列的系统发育分析表明，菌株AH-1是希内拉属（Shinella）的成员，与暗褐希内拉（Shinella fusca）DC-196（97.7%）、颗粒希内拉（Shinella granuli）Ch06（97.3%）、大田希内拉（Shinella daejeonensis）MJ02（97.1%）和八重山希内拉（Shinella yambaruensis）MS4（96.8%）的序列相似性最高，与其他已知希内拉属物种的序列相似性较低（<96.7%）。化学分类分析表明，菌株AH-1以Q-10作为主要类异戊二烯醌；特征性总和8（C18:1ω6c/C18:1ω7c）、C16:0、C12:0醛、C18:0、C19:0环ω8c和C18:0 3-OH为主要脂肪酸。菌株AH-1与暗褐希内拉DC-196（DNA-DNA杂交率为28.6±5.7%）、颗粒希内拉Ch06（43.7±3.8%）和大田希内拉MJ02（48.1±2.6%）的DNA-DNA相关性较低。DNA G+C含量为68.2 mol%。基于系统发育和表型特征、化学分类数据以及DNA-DNA杂交结果，菌株AH-1被认为是希内拉属的一个新物种，为此提出新名称污染土壤希内拉（Shinella pollutisoli）sp. nov.（模式菌株AH-1=KCTC 52677=CCTCC AB 2017242）。
