Shinella pollutisoli sp. nov., isolated from tetrabromobisphenol A-contaminated soil.

Strain AH-1, a Gram-negative, aerobic, non-spore-forming, motile, rod-shaped bacterium, was isolated from tetrabromobisphenol A-contaminated soil in China. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AH-1 was a member of the genus Shinella and showed the highest sequence similarity to Shinella fusca DC-196 (97.7 %), Shinella granuli Ch06 (97.3 %), Shinella daejeonensis MJ02 (97.1 %) and Shinella yambaruensis MS4 (96.8 %), and lower (<96.7 %) sequence similarity to other known Shinella species. Chemotaxonomic analysis revealed that strain AH-1 possessed Q-10 as the major isoprenoid quinone; and summed feature 8 (C18 : 1ω6c/C18 : 1ω7c), C16 : 0, C12 : 0 aldehyde, C18 : 0, C19 : 0 cyclo ω8c and C18 : 0 3-OH were the predominant fatty acids. Strain AH-1 showed low DNA-DNA relatedness to S. fusca DC-196 (28.6±5.7 %), S. granuli Ch06 (43.7±3.8 %) and S. daejeonensis MJ02 (48.1±2.6 %). The DNA G+C content was 68.2 mol%. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data and DNA-DNA hybridization, strain AH-1 is considered a novel species of the genus Shinella, for which the name Shinella pollutisoli sp. nov. (type strain AH-1=KCTC 52677=CCTCC AB 2017242) is proposed.