Guinan P, Shaw M, Targonski P, Ray V, Rubenstein M
Hektoen Institute for Medical Research, Cook County Hospital, Chicago IL 60612.
J Surg Oncol. 1989 Nov;42(3):175-80. doi: 10.1002/jso.2930420309.
Cytokeratins are intermediate filaments found within basal and secretory epithelial cells. Antisera raised against cytokeratins are available but frequently differ in specificity. Many are incompletely characterized for their reactivity against epithelial components. Cytokeratin (Cyto) P is a polyclonal antisera specific for 56 and 64 kd cytokeratins. Cyto M is a pool of monoclonals reacting against 40, 46, 50, 52, 58, and 65-67 kd cytokeratins. Initially, utilizing immunohistologic techniques, we evaluated these two antisera for their ability to distinguish between prostatic tissues of benign (benign prostatic hypertrophy [BPH]) or malignant (carcinoma of the prostate [CAP]) origin in the 34 cases evaluated. Specimens were analyzed for both Cyto P and Cyto M reactivity, as well as for the degree of reactivity. Lastly, in an effort to determine the morphologic relationship of atypical hyperplasia (AH) with either BPH or CAP, nine additional prostate specimens were analyzed. Cyto P was reactive in 8 of 8 (100%) BPH specimens and in 2 of 26 (8%) CAP specimens. Mean Cyto P degree of reactivity in the positive specimens was greater in BPH than in CAP (2.6 vs. 1.0). Cyto M reactivity was present in 8 of 8 (100%) BPH specimens and in 23 of 25 (92%) CAP specimens. Mean Cyto M degree of reactivity in the positive specimens was greater in CAP than in BPH (3.6 vs. 2.8). Cyto P was reactive in 3 of 9 (33%) AH specimens, with a mean degree of reactivity of 2.7. Cyto M was reactive in 9 of 9 (100%) AH specimens, with a mean degree of reactivity of 3.9. Cyto P reacted with only the basal cells, whereas Cyto M reacted with basal as well as secretory cells. These differences appeared to be the result of the differential reactivity of basal cells, which are present in BPH but absent in CAP. In summary, Cyto P and Cyto M are potentially useful markers in differentiating BPH from CAP, and it appears that AH is immunohistopathologically related to both.
细胞角蛋白是存在于基底和分泌上皮细胞内的中间丝。针对细胞角蛋白制备的抗血清是可用的，但特异性常常不同。许多抗血清针对上皮成分的反应性特征不完全明确。细胞角蛋白（Cyto）P是一种针对56和64kd细胞角蛋白的多克隆抗血清。Cyto M是一组针对40、46、50、52、58以及65 - 67kd细胞角蛋白的单克隆抗体混合物。最初，我们利用免疫组织学技术，在34例评估病例中，评估这两种抗血清区分良性（良性前列腺增生[BPH]）或恶性（前列腺癌[CAP]）起源的前列腺组织的能力。对标本进行Cyto P和Cyto M反应性分析以及反应程度分析。最后，为了确定非典型增生（AH）与BPH或CAP的形态学关系，又分析了另外9例前列腺标本。Cyto P在8例（100%）BPH标本中的8例有反应，在26例CAP标本中的2例有反应。阳性标本中Cyto P的平均反应程度在BPH中高于CAP（2.6对1.0）。Cyto M反应性在8例（100%）BPH标本中的8例存在，在25例CAP标本中的23例（92%）存在。阳性标本中Cyto M的平均反应程度在CAP中高于BPH（3.6对2.8）。Cyto P在9例AH标本中的3例（33%）有反应，平均反应程度为2.7。Cyto M在9例AH标本中的9例（100%）有反应，平均反应程度为3.9。Cyto P仅与基底细胞反应，而Cyto M与基底细胞以及分泌细胞都反应。这些差异似乎是由于基底细胞反应性不同导致的，基底细胞存在于BPH中而不存在于CAP中。总之，Cyto P和Cyto M在区分BPH和CAP方面可能是有用的标志物，并且AH在免疫组织病理学上似乎与两者都相关。
