Kozub N A, Boguslavskiĭ R L, Sozinov I A, Tverdokhleb E V, Ksinias I N, Blium Ia B, Sozinov A A
Tsitol Genet. 2014 Jan-Feb;48(1):41-51.
Variation at eight storage protein loci was analyzed in the collection of T. spelta accessions from the National Centre of Plant Genetic Resources of Ukraine, most of which are European spelts. The analysis allowed identification of seven alleles at the Gli-B1 locus, five alleles at the Gli-A1 and Glu-B1 loci, three alleles at the Gli-A3 locus, two at the Gli-D1, Gli-B5, Glu-A1, and Glu-D1 loci. The majority of alleles are encountered among common wheat cultivars, only five alleles were specific for spelts. The high frequency of the alleles Gli-B1hs* and h encoding the 45-type gamma-gliadin in European spelts and durum wheat cultivars, as well as the occurrence of these alleles in T. dicoccum, in particular, in accessions from Switzerland and Germany, supports von Büren's hypothesis that European spelt resulted from hybridization between a tetraploid wheat with the 45-type y-gliadin and a hexaploid wheat. Analysis of genetic distances based on the genotypes at eight storage protein loci permitted differentiation of the Asian spelt accession from European spelts.
对来自乌克兰国家植物遗传资源中心的斯佩尔特小麦种质资源库中的材料进行了8个贮藏蛋白位点的变异分析,其中大部分是欧洲斯佩尔特小麦。分析发现,Gli-B1位点有7个等位基因,Gli-A1和Glu-B1位点有5个等位基因,Gli-A3位点有3个等位基因,Gli-D1、Gli-B5、Glu-A1和Glu-D1位点有2个等位基因。大多数等位基因在普通小麦品种中出现,只有5个等位基因是斯佩尔特小麦特有的。欧洲斯佩尔特小麦和硬粒小麦品种中Gli-B1hs*和编码45型γ-醇溶蛋白的h等位基因频率较高,以及这些等位基因在二粒小麦中出现,特别是在来自瑞士和德国的种质中出现,支持了冯·比伦的假说,即欧洲斯佩尔特小麦是由具有45型γ-醇溶蛋白的四倍体小麦与六倍体小麦杂交产生的。基于8个贮藏蛋白位点的基因型分析遗传距离,可将亚洲斯佩尔特小麦种质与欧洲斯佩尔特小麦区分开来。