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利用Gli-1和Glu-3位点的等位基因特异性引物进行PCR分析普通小麦品种和近等基因系

[Analysis of common wheat varieties and near-isogenic lines by PCR with allele-specific primers for Gli-1 and Glu-3 loci].

作者信息

Polishchuk A M, Chebotar S V, Blahodarova O M, Kozub N O, Sozinov I O, Syvolap Iu M

出版信息

Tsitol Genet. 2010 Nov-Dec;44(6):22-31.

PMID:21254616
Abstract

The allelic characteristics of the Gli-A1, Gli-B1, Gli-D1 and Glu-A3 loci of 14 bread wheat varieties and 6 near-isogenic lines derived from Bezostaya 1 have been detected by PCR analysis. The conformity of molecular-genetic data and electrophoresis of storage proteins has been determined: the allelic variants of gliadins Gli-A1o and Gi-A1m correspond to the PCR-allele GliA1.2, the gliadin variants Gli-A1f, Gli-A1b, Gli-A1c correspond to the PCR-allele GliA 1.1, the allelic variants Gli-B1b, Gli-B1d--to the PCR-allele GliB1.1 and the variants Gli-B1e, Gli-B1g, Gli-B1c-to the PCR-allele GliB1.2. A new PCR-allele at the GliB) locus in the line Gli-B1-12 (with the gliadin block Gli-B1o from Levent) was identified.

摘要

通过PCR分析检测了14个面包小麦品种以及源自别佐斯塔亚1号的6个近等基因系的Gli-A1、Gli-B1、Gli-D1和Glu-A3位点的等位基因特征。确定了分子遗传数据与贮藏蛋白电泳的一致性:醇溶蛋白Gli-A1o和Gi-A1m的等位基因变体对应于PCR等位基因GliA1.2,醇溶蛋白变体Gli-A1f、Gli-A1b、Gli-A1c对应于PCR等位基因GliA 1.1,等位基因变体Gli-B1b、Gli-B1d对应于PCR等位基因GliB1.1,变体Gli-B1e、Gli-B1g、Gli-B1c对应于PCR等位基因GliB1.2。在Gli-B1-12系(具有来自莱文特的醇溶蛋白块Gli-B1o)的GliB)位点鉴定出一个新的PCR等位基因。

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