Mdivani T V, Agladze A G, Tumanishvili G D
Tsitol Genet. 1989 Jul-Aug;23(4):3-6.
Nucleolar ultrastructure of the rat hepatocytes after bilateral adrenalectomy and cortisol stimulation has been studied by the electron-microscopic method Traits of nucleolar inactivation (a decrease of granular component enlargement of fibrillar centres, condensation of peri- and intranucleolar chromatin, etc.) are observed in hepatocyte nucleoli 5 days after adrenalectomy. Cortisol stimulation of hepatocytes of the adrenalectomized rats leads to nucleolar activation (4h, 5h, 8h after the hormone injection). Adrenalectomy with following cortisol injection is a useful model to study inactivation and activation of ribosomal genes in the target cells.
采用电子显微镜方法研究了双侧肾上腺切除及皮质醇刺激后大鼠肝细胞的核仁超微结构。肾上腺切除术后5天，在肝细胞核仁中观察到核仁失活的特征（颗粒成分减少、纤维中心增大、核仁周围及核仁内染色质凝聚等）。对肾上腺切除大鼠的肝细胞进行皮质醇刺激会导致核仁激活（注射激素后4小时、5小时、8小时）。肾上腺切除后注射皮质醇是研究靶细胞中核糖体基因失活和激活的有用模型。
