用于N-糖蛋白质组分析的定量糖蛋白质组学

Quantitative glycoproteomics for N-glycoproteome profiling.

作者信息

Pan Sheng

机构信息

Department of Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA, 98195, USA,

出版信息

Methods Mol Biol. 2014;1156:379-88. doi: 10.1007/978-1-4939-0685-7_25.

DOI:10.1007/978-1-4939-0685-7_25

PMID:24792002

Abstract

Glycoproteomics, the global analysis of glycoproteins, is a subfield of proteomics with high biological and clinical relevance. Glycoproteomics integrates knowledge and techniques drawing from glycotechnology, separation science, mass spectrometry, and bioinformatics to support systematic identification and quantification of glycoproteins in a complex biological sample. In this chapter, we describe a shotgun proteomics-based glycoproteomics platform that provides precise mapping of protein N-glycosylation sites and quantitative assessment of glycosylation site occupancy on a global scale.

摘要

糖蛋白质组学，即对糖蛋白进行全面分析，是蛋白质组学的一个子领域，具有高度的生物学和临床相关性。糖蛋白质组学整合了来自糖技术、分离科学、质谱和生物信息学的知识与技术，以支持对复杂生物样品中糖蛋白进行系统的鉴定和定量分析。在本章中，我们描述了一种基于鸟枪法蛋白质组学的糖蛋白质组学平台，该平台能够在全球范围内提供蛋白质N-糖基化位点的精确图谱以及糖基化位点占有率的定量评估。