Thaysen-Andersen Morten, Packer Nicolle H, Schulz Benjamin L
From the ‡Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia;
§School of Chemistry & Molecular Biosciences, St Lucia, The University of Queensland, Brisbane, QLD, Australia.
Mol Cell Proteomics. 2016 Jun;15(6):1773-90. doi: 10.1074/mcp.O115.057638. Epub 2016 Feb 29.
The glycoproteome remains severely understudied because of significant analytical challenges associated with glycoproteomics, the system-wide analysis of intact glycopeptides. This review introduces important structural aspects of protein N-glycosylation and summarizes the latest technological developments and applications in LC-MS/MS-based qualitative and quantitative N-glycoproteomics. These maturing technologies provide unique structural insights into the N-glycoproteome and its synthesis and regulation by complementing existing methods in glycoscience. Modern glycoproteomics is now sufficiently mature to initiate efforts to capture the molecular complexity displayed by the N-glycoproteome, opening exciting opportunities to increase our understanding of the functional roles of protein N-glycosylation in human health and disease.
由于完整糖肽的全系统分析即糖蛋白质组学面临重大分析挑战，糖蛋白质组仍未得到充分研究。本综述介绍了蛋白质N-糖基化的重要结构方面，并总结了基于液相色谱-串联质谱的定性和定量N-糖蛋白质组学的最新技术发展及应用。这些不断成熟的技术通过补充糖科学中的现有方法，为N-糖蛋白质组及其合成与调控提供了独特的结构见解。现代糖蛋白质组学现已足够成熟，可着手努力捕捉N-糖蛋白质组所呈现的分子复杂性，为增进我们对蛋白质N-糖基化在人类健康和疾病中的功能作用的理解带来了令人兴奋的机遇。
