Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida; Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida.
Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida; Anne Bates Leach Eye Hospital, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida.
Am J Ophthalmol. 2014 Jul;158(1):64-70.e2. doi: 10.1016/j.ajo.2014.04.007. Epub 2014 Apr 29.
To compare the in vitro effect of rose bengal and riboflavin as photosensitizing agents for photodynamic therapy (PDT) on fungal isolates that are common causes of fungal keratitis.
Experimental study.
Three isolates (Fusarium solani, Aspergillus fumigatus, Candida albicans) recovered from patients with confirmed fungal keratitis were used in the experiments. Isolates were grown on Sabouraud-Dextrose agar, swabbed, and prepared in suspension, and 1 mL aliquots were inoculated onto test plates in triplicate. Test plates were separated into 5 groups: Group 1, no treatment; Group 2, 0.1% rose bengal alone; Group 3, 518 nm irradiation alone; Group 4, riboflavin PDT (riboflavin + 375 nm irradiation); and Group 5, rose bengal PDT (rose bengal + 518 nm irradiation). Irradiation was performed over a circular area using either a green light-emitting diode (LED) array (peak wavelength: 518 nm) or an ultraviolet-A LED array (peak wavelength: 375 nm). Test plates were irradiated with an energy density of 5.4 J/cm(2). Later, plates were placed in a 30 C incubator and observed for growth.
Rose bengal-mediated PDT successfully inhibited the growth of all 3 fungal isolates in the irradiated area. All other groups exhibited unrestricted growth throughout the plate.
Rose bengal-mediated PDT successfully inhibited the growth of 3 types of fungi. No other experimental groups, including riboflavin-mediated PDT, had any inhibitory effect on the isolates. The results might be useful for the treatment of patients suffering from corneal infection.
比较孟加拉玫瑰红和核黄素作为光动力疗法（PDT）光敏剂对常见真菌性角膜炎病原体的体外抑制效果。
实验研究。
从确诊为真菌性角膜炎的患者中分离出三种真菌（茄病镰刀菌、烟曲霉、白色念珠菌），用于本实验。将分离株在沙氏葡萄糖琼脂上生长，然后进行擦拭和悬浮液制备，将 1 mL 等分试样接种到三个重复的测试平板中。将测试平板分为 5 组：第 1 组，无处理；第 2 组，单独使用 0.1%孟加拉玫瑰红；第 3 组，单独使用 518nm 光照射；第 4 组，核黄素 PDT（核黄素+375nm 光照射）；第 5 组，孟加拉玫瑰红 PDT（孟加拉玫瑰红+518nm 光照射）。使用绿光发光二极管（LED）阵列（峰值波长：518nm）或紫外线-A LED 阵列（峰值波长：375nm）在圆形区域内进行照射。测试板以 5.4J/cm2 的能量密度进行照射。随后，将平板置于 30°C 孵育箱中并观察生长情况。
孟加拉玫瑰红介导的 PDT 成功抑制了照射区域内所有 3 种真菌分离株的生长。其他所有组在整个平板上均表现出不受限制的生长。
孟加拉玫瑰红介导的 PDT 成功抑制了 3 种真菌的生长。其他实验组（包括核黄素介导的 PDT）对分离株没有任何抑制作用。这些结果可能对治疗角膜感染患者有用。
