Zhang Jing, Lei Du, Ma Jun, Wang Mengxing, Shen Guohua, Wang Hui, Yang Guang, Du Xiaoxia
Shanghai Key Laboratory of Magnetic Resonance, Department of Physics, East China Normal University, 3663 North Zhong-Shan Road, Shanghai, 200062, People's Republic of China.
Neurochem Res. 2014 Jul;39(7):1355-62. doi: 10.1007/s11064-014-1320-4. Epub 2014 May 3.
Nocturnal enuresis is a common developmental disorder in children; primary monosymptomatic nocturnal enuresis (PMNE) is the dominant subtype. Previous literature has suggested that the prefrontal cortex and the pons are both involved in micturition control. This study aimed to investigate the metabolic levels of the left prefrontal cortex and the pons in children with PMNE by proton magnetic resonance spectroscopy (1H-MRS). Twenty-five children with PMNE and 25 healthy children took part in our experiments. Magnetic resonance examinations were performed on a Siemens 3T Trio Tim scanner. For each subject, localized 1H-MRS was acquired from the left prefrontal cortex (mainly in brodmann area 9) and the pons with a point-resolved spectroscopy sequence with repetition time 2,000 ms, echo time 30 ms and 64 averages. The LCModel software package was used to analyze the MRS raw data, and two-sample t tests were used to determine significant differences between the two groups. The results revealed a significant reduction in metabolite to total creatine ratios of N-acetylaspartate (NAA/tCr) in the left prefrontal cortex and the pons for children with PMNE compared to healthy children. Our study suggests that metabolism is disturbed in the prefrontal cortex and the pons in children with PMNE, which may be associated with the symptoms of enuresis.
夜间遗尿症是儿童常见的发育障碍；原发性单症状性夜间遗尿症（PMNE）是主要亚型。既往文献表明，前额叶皮质和脑桥均参与排尿控制。本研究旨在通过质子磁共振波谱（1H-MRS）研究PMNE患儿左侧前额叶皮质和脑桥的代谢水平。25例PMNE患儿和25例健康儿童参与了我们的实验。在西门子3T Trio Tim扫描仪上进行磁共振检查。对每个受试者，采用点分辨波谱序列，重复时间2000 ms，回波时间30 ms，采集64次平均信号，从左侧前额叶皮质（主要位于布罗德曼9区）和脑桥获取局部1H-MRS。使用LCModel软件包分析MRS原始数据，并采用两样本t检验确定两组之间的显著差异。结果显示，与健康儿童相比，PMNE患儿左侧前额叶皮质和脑桥中N-乙酰天门冬氨酸与总肌酸的代谢物比率（NAA/tCr）显著降低。我们的研究表明，PMNE患儿前额叶皮质和脑桥的代谢受到干扰，这可能与遗尿症状有关。
