Myrseth L E, Hagve T A, Prydz H
Research Institute for Internal Medicine, University of Oslo, Norway.
Anal Biochem. 1989 Aug 15;181(1):86-9. doi: 10.1016/0003-2697(89)90397-7.
A method for separation and visualization of the different apolipoprotein B species using 0.2% sodium dodecyl sulfate-1.5% agarose gel electrophoresis and immunoblotting is described. The method is capable of demonstrating the different forms of apolipoprotein B (apo B) in plasma volumes of 10-50 microliters without prior ultracentrifugation. After ultracentrifugation of samples, estimation of the ratio between apo B 48 and apo B 100 is possible by scanning of Coomassie-stained gels or immunoblots.
