Ichimura M, Okamoto M, Mori S, Tohge H, Ochi K, Harada H
School of Health Sciences, Medical School, Okayama University, Japan.
Electrophoresis. 1998 May;19(5):687-91. doi: 10.1002/elps.1150190514.
In an attempt to subfractionate apolipoprotein B-containing lipoproteins (apoB-Lp), agarose isoelectric focusing (IEF) of apoB-Lp in human sera was carried out in the presence of two kinds of cyclodextrins (CDs), which have different affinities for cholesterol. ApoB-Lp in the agarose gels was detected by immunofixation, followed by protein staining. In the presence of both 11.4 mg/mL alpha-CD and 3.51 mg/mL beta-CD, but not either one alone, IEF separated a maximum of six apoB-Lp bands. Electrophoresis failed to separate the apoB-Lp subfractions under similar conditions with CDs. The patterns of apoB-Lp in IEF with the CDs appeared to differ in some sera. Since the subfractions of apoB-Lp can be separated without ultracentrifugation of serum, the present method serves for the screening of apoB-Lp heterogeneity on native serum lipoproteins.
为了对含载脂蛋白B的脂蛋白（apoB-Lp）进行亚组分分离，在两种对胆固醇亲和力不同的环糊精（CDs）存在的情况下，对人血清中的apoB-Lp进行了琼脂糖等电聚焦（IEF）。通过免疫固定法检测琼脂糖凝胶中的apoB-Lp，然后进行蛋白质染色。在同时存在11.4 mg/mL的α-CD和3.51 mg/mL的β-CD时，而非单独使用其中任何一种时，IEF最多可分离出六条apoB-Lp条带。在类似的含CDs条件下，电泳未能分离出apoB-Lp亚组分。在IEF中使用CDs时，不同血清中apoB-Lp的模式似乎有所不同。由于无需对血清进行超速离心即可分离apoB-Lp的亚组分，因此本方法可用于筛选天然血清脂蛋白上apoB-Lp的异质性。
