Wekerle L, Sarlós P
Forschungszentrum für Tierproduktion und Tierernährung Gödöllö - Herceghalom/Hungary.
Andrologia. 1989 Sep-Oct;21(5):479-82.
303 semen smear from the ejaculates of 101 boars were prepared with 3 different staining techniques. The staining methods were: the Cerovsky, the Farelly, the Wells-Awa. Native semen smear were examined by phase contrast microscope. The examined sperm abnormalities were the following: acrosomal anomalies, cytoplasmatic droplets (proximal and distal), tail anomalies (hairpin curved, single bent, coiled tails) and head anomalies. The phase contrast microscope technique have not found useful for detecting of acrosome and head anomalies. The method we have found most useful for staining the acrosome was the Wells-Awa, but this staining technique was not suitable for detecting of cytoplasmatic droplets. Farelly and Cerovsky staining technique have appeared highly desirable for staining of sperm abnormalities. The Cerovsky staining technique was proved most useful in everyday veterinary practice, as it needs very short time and shows the same rate in detecting acrosome anomalies than the Wells-Awa staining technique.
用3种不同的染色技术制备了101头公猪精液的303份精液涂片。染色方法为：塞罗夫斯基法、法雷利法、韦尔斯 - 阿瓦法。用相差显微镜检查未染色的精液涂片。检查的精子异常情况如下：顶体异常、细胞质滴（近端和远端）、尾部异常（发夹弯曲、单弯、螺旋尾）和头部异常。相差显微镜技术对于检测顶体和头部异常没有用处。我们发现对顶体染色最有用的方法是韦尔斯 - 阿瓦法，但这种染色技术不适用于检测细胞质滴。法雷利法和塞罗夫斯基染色技术对于精子异常染色似乎非常理想。塞罗夫斯基染色技术在日常兽医实践中被证明是最有用的，因为它所需时间非常短，并且在检测顶体异常方面与韦尔斯 - 阿瓦染色技术的检出率相同。
