ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, Av. Carl Friedrich Gauss, 3, 08860 Castelldefels (Barcelona), Spain.
ICFO-Institut de Ciencies Fotoniques, Mediterranean Technology Park, Av. Carl Friedrich Gauss, 3, 08860 Castelldefels (Barcelona), Spain.
Curr Opin Chem Biol. 2014 Jun;20:22-8. doi: 10.1016/j.cbpa.2014.04.005. Epub 2014 May 8.
Super-resolution microscopy is an enabling technology that allows biologists to visualize cellular structures at nanometer length scales using far-field optics. To break the diffraction barrier, it is necessary to leverage the distinct molecular states of fluorescent probes. At the same time, the existence of these different molecular states and the photophysical properties of the fluorescent probes can complicate data quantification and interpretation. Here, we review the pitfalls in super-resolution data analysis that must be avoided for proper interpretation of images.
超分辨率显微镜是一种使能技术，它允许生物学家使用远场光学在纳米长度尺度上可视化细胞结构。为了突破衍射障碍，有必要利用荧光探针的独特分子状态。同时，这些不同的分子状态的存在和荧光探针的光物理性质可能会使数据的定量和解释复杂化。在这里，我们回顾了在超分辨率数据分析中必须避免的陷阱，以便正确解释图像。
