Perrine S P, Miller B A, Morgan D A
Department of Neoplastic Diseases, Hahnemann University, Philadelphia, PA.
Biochem Biophys Res Commun. 1989 Oct 31;164(2):857-62. doi: 10.1016/0006-291x(89)91537-4.
The recently described MB-02 human cell line requires Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) for continuous growth and terminally differentiates into enucleate, hemoglobinized cells in response to erythropoietin. Here, analysis of globin production now demonstrates that uninduced MB-02 cells produce alpha globin and the fetal globin chains G gamma and A gamma in a ratio of 1:3. Addition of erythropoietin results in de novo synthesis of beta globin chains and a marked increase in total Hb/cell. Thus, the MB-02 cell line partially recapitulates the fetal to adult globin switch that occurs during erythroid and human fetal development and provides a new clonal human erythroid progenitor system for investigating the biochemical and molecular events involved in globin gene switching.
最近描述的MB - 02人细胞系需要粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）来持续生长，并在促红细胞生成素的作用下终末分化为无核、血红蛋白化的细胞。在此，对珠蛋白生成的分析表明，未诱导的MB - 02细胞以1:3的比例产生α珠蛋白以及胎儿珠蛋白链Gγ和Aγ。添加促红细胞生成素会导致β珠蛋白链的从头合成以及每个细胞中总血红蛋白的显著增加。因此，MB - 02细胞系部分重现了在红系和人类胎儿发育过程中发生的从胎儿型到成人型珠蛋白的转换，并为研究参与珠蛋白基因转换的生化和分子事件提供了一个新的克隆人红系祖细胞系统。
