Grange Cristina, Moggio Aldo, Tapparo Marta, Porta Stefano, Camussi Giovanni, Bussolati Benedetta
Department of Medical Sciences, University of Torino, Torino, Italy.
Physiol Rep. 2014 May 2;2(5):e12009. doi: 10.14814/phy2.12009. Print 2014.
Abstract Recent approaches of regenerative medicine can offer a therapeutic option for patients undergoing acute kidney injury. In particular, mesenchymal stem cells were shown to ameliorate renal function and recovery after acute damage. We here evaluated the protective effect and localization of CD133(+) renal progenitors from the human inner medulla in a model of glycerol-induced acute tubular damage and we compared the results with those obtained with bone marrow-derived mesenchymal stem cells. We found that CD133(+) progenitor cells promoted the recovery of renal function, preventing tubular cell necrosis and stimulating resident cell proliferation and survival, similar to mesenchymal stem cells. In addition, by optical imaging analysis, CD133(+) progenitor cells accumulated within the renal tissue, and a reduced entrapment in lung, spleen, and liver was observed. Mesenchymal stem cells were detectable at similar levels in the renal tissue, but a higher signal was present in extrarenal organs. Both cell types produced several cytokines/growth factors, suggesting that a combination of different mediators is involved in their biological action. These results indicate that human CD133(+) progenitor cells are renotropic and able to improve renal regeneration in acute kidney injury.
摘要 再生医学的最新方法可为急性肾损伤患者提供一种治疗选择。特别是，间充质干细胞已被证明可改善急性损伤后的肾功能和恢复情况。我们在此评估了来自人肾内髓的CD133(+)肾祖细胞在甘油诱导的急性肾小管损伤模型中的保护作用和定位，并将结果与骨髓来源的间充质干细胞进行了比较。我们发现，CD133(+)祖细胞促进肾功能恢复，防止肾小管细胞坏死，并刺激驻留细胞增殖和存活，这与间充质干细胞相似。此外，通过光学成像分析，CD133(+)祖细胞在肾组织内积聚，且在肺、脾和肝中的滞留减少。间充质干细胞在肾组织中的检测水平相似，但肾外器官中存在较高信号。两种细胞类型均产生多种细胞因子/生长因子，表明不同介质的组合参与了它们的生物学作用。这些结果表明，人CD133(+)祖细胞具有肾趋向性，能够改善急性肾损伤中的肾再生。
