Ohtsuki T, Yawata Y, Wada H, Sugihara T, Mori M, Namba M
Department of Medicine, Kawasaki Medical School, Kurashiki, Japan.
Br J Haematol. 1989 Oct;73(2):199-204. doi: 10.1111/j.1365-2141.1989.tb00252.x.
Two human myeloma cell lines, KMS-12-PE and KMS-12-BM, were established from a 64-year-old woman with a non-producing type of multiple myeloma. The KMS-12-PE line originated from the pleural effusion and the KMS-12-BM from the bone marrow. These two lines showed the same chromosome marker, t(11:14)(q13:q32). However, their phenotypes of surface markers differed from each other. KMS-12-BM cells were positive to CD20, CD38 and PCA-1. showing the plasmacytoid (immature plasma cell) stage of B-cell differentiation, while KMS-12-PE cells were positive to CD38 and PCA-1, but not to CD20, indicating the terminal differentiated stage of B-cells. As seen in the pleural effusion of the patient. KMS-12-PE cells ectopically produced a salivary type of amylase, but KMS-12-BM cells did not. Interestingly, the chromosome abnormality of del(1)(p22----pter) near the region of 1p21, where the amylase gene was assigned, was noticed in as many as 76% of KMS-12-PE cells.
两种人骨髓瘤细胞系，KMS - 12 - PE和KMS - 12 - BM，是从一名64岁的非分泌型多发性骨髓瘤女性患者中建立的。KMS - 12 - PE细胞系源自胸腔积液，KMS - 12 - BM细胞系源自骨髓。这两种细胞系显示出相同的染色体标记，即t(11:14)(q13:q32)。然而，它们的表面标志物表型彼此不同。KMS - 12 - BM细胞对CD20、CD38和PCA - 1呈阳性，显示B细胞分化的浆细胞样（未成熟浆细胞）阶段，而KMS - 12 - PE细胞对CD38和PCA - 1呈阳性，但对CD20呈阴性，表明是B细胞的终末分化阶段。如在患者的胸腔积液中所见，KMS - 12 - PE细胞异位产生一种唾液型淀粉酶，但KMS - 12 - BM细胞不产生。有趣地是，在多达76%的KMS - 12 - PE细胞中发现了靠近淀粉酶基因所在的1p21区域的1号染色体缺失（del(1)(p22----pter)）异常。
