College of Ocean and Earth Science, Xiamen University, Xiamen 361005, PR China; State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China.
State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of State Oceanic Administration & Fujian, Third Institute of Oceanography, Xiamen 361005, PR China.
Fish Shellfish Immunol. 2014 Jul;39(1):17-23. doi: 10.1016/j.fsi.2014.04.011. Epub 2014 May 2.
In this study, we identified three white spot syndrome virus (WSSV) strains (WSSV-CN01, WSSV-CN02 and WSSV-CN03) with significant differences in virulence. Among them, WSSV-CN01 caused significant higher and earlier mortality in redclaw crayfish Cherax quadricarinatus, thus was determined as high-virulent, while WSSV-CN02 and WSSV-CN03 were moderate-virulent and low-virulent. By investigating the total number of the circulating haemocytes and the activity of immune relative enzymes, we demonstrated that the different virulent WSSV strains induced distinct immune response in the host. Notably, a dramatic reduction of circulating haemocytes was observed in the crayfish infected with WSSV-CN01 and WSSV-CN02 but not WSSV-CN03. Further analysis revealed that cell death induced by WSSV-CN01 and WSSV-CN02 might be responsible for the decrease of circulating haemocytes.
在这项研究中，我们鉴定了三种具有显著毒力差异的白斑综合征病毒（WSSV）株（WSSV-CN01、WSSV-CN02 和 WSSV-CN03）。其中，WSSV-CN01 导致罗氏沼虾（Cherax quadricarinatus）的死亡率显著更高且更早，因此被确定为高毒力株，而 WSSV-CN02 和 WSSV-CN03 则为中毒力和低毒力株。通过调查循环血细胞总数和免疫相关酶的活性，我们证明了不同毒力的 WSSV 株在宿主中诱导了不同的免疫反应。值得注意的是，感染 WSSV-CN01 和 WSSV-CN02 的罗氏沼虾中观察到循环血细胞数量的急剧减少，但感染 WSSV-CN03 的罗氏沼虾中则没有这种情况。进一步的分析表明，WSSV-CN01 和 WSSV-CN02 诱导的细胞死亡可能是导致循环血细胞减少的原因。
