Li Fang, Gao Meiling, Xu Limei, Yang Feng
Key Laboratory of Marine Genetic Resources, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China.
Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China.
Virus Genes. 2017 Apr;53(2):249-258. doi: 10.1007/s11262-016-1421-z. Epub 2016 Dec 24.
Three white spot syndrome virus (WSSV) isolates of different virulence were identified in our previous study, the high-virulent strain WSSV-CN01, the moderate-virulent strain WSSV-CN02 and the low-virulent strain WSSV-CN03. In this study, the genomes of these three WSSV isolates were sequenced, annotated and compared. The genome sizes for WSSV-CN01, WSSV-CN02, and WSSV-CN03 are 309,286, 294,261, and 284,148 bp, bearing 177, 164, and 154 putative protein-coding genes, respectively. The genomic variations including insertions, deletions, and substitutions were investigated. Thirty four genes show >20% variation in their sequences in WSSV-CN02 or WSSV-CN03, in comparison with WSSV-CN01, including six envelope protein genes (wsv237/vp41A, wsv238/vp52A, wsv338/vp62, wsv339/vp39, wsv077/vp36A, and wsv242/vp41B), and two immediate-early genes (wsv108 and wsv178). The genomic variations among WSSV isolates of different virulence, especially those in the coding regions, certainly provide new insight into the understanding of the molecular basis of WSSV pathogenesis.
在我们之前的研究中鉴定出了三种不同毒力的白斑综合征病毒（WSSV）分离株，即高毒力株WSSV-CN01、中毒力株WSSV-CN02和低毒力株WSSV-CN03。在本研究中，对这三种WSSV分离株的基因组进行了测序、注释和比较。WSSV-CN01、WSSV-CN02和WSSV-CN03的基因组大小分别为309,286、294,261和284,148 bp，分别含有177、164和154个推定的蛋白质编码基因。研究了包括插入、缺失和替换在内的基因组变异。与WSSV-CN01相比，有34个基因在WSSV-CN02或WSSV-CN03中的序列变异超过20%，包括6个包膜蛋白基因（wsv237/vp41A、wsv238/vp52A、wsv338/vp62、wsv339/vp39、wsv077/vp36A和wsv242/vp41B）以及2个立即早期基因（wsv108和wsv178）。不同毒力的WSSV分离株之间的基因组变异，尤其是编码区的变异，无疑为理解WSSV发病机制的分子基础提供了新的见解。
