Mackie R M, Clelland D B, Skerrow C J
Department of Dermatology, University of Glasgow, Scotland.
J Clin Pathol. 1989 Nov;42(11):1173-7. doi: 10.1136/jcp.42.11.1173.
The presence of both laminin and type IV collagen was sought at the dermo-epidermal junction and in the dermis adjacent to benign melanocytic naevi of the junctional, compound, and intradermal types; dysplastic naevi; and both primary and secondary melanoma. In all, 154 lesions were studied, using antibodies to laminin and type IV collagen and an indirect immunoperoxidase technique. The staining patterns seen with the two antibodies were virtually identical, although that of laminin was generally fainter. Breaks in and thinning of the normally continuous line of type IV collagen and laminin at the dermo-epidermal junction were seen in association with the junctional activity of benign naevi, and in malignant melanomas in association with invasive tumour cells. Both benign and malignant cells of the melanocyte series showed relatively light pericellular staining around individual cells and clusters of cells in the papillary dermis. This staining pattern was much stronger in the deeper reticular dermis. It is concluded that the pattern of staining of these two antibodies and in particular the presence of breaks in type IV collagen and laminin at the dermo-epidermal junction are not specific for either benign or malignant melanocytic lesions and cannot be used as a diagnostic marker of invasive malignancy.
在交界痣、混合痣和皮内痣等良性黑素细胞痣、发育异常痣以及原发性和继发性黑色素瘤的真皮 - 表皮交界处及相邻真皮中，探寻层粘连蛋白和IV型胶原的存在情况。总共对154个病变进行了研究，采用针对层粘连蛋白和IV型胶原的抗体以及间接免疫过氧化物酶技术。尽管层粘连蛋白的染色通常较淡，但两种抗体所见的染色模式几乎相同。在真皮 - 表皮交界处，IV型胶原和层粘连蛋白正常连续线的中断和变薄与良性痣的交界活性相关，在恶性黑色素瘤中则与侵袭性肿瘤细胞相关。黑素细胞系列的良性和恶性细胞在乳头真皮中单个细胞和细胞簇周围均显示相对较淡的细胞周染色。这种染色模式在更深的网状真皮中要强得多。结论是，这两种抗体的染色模式，特别是真皮 - 表皮交界处IV型胶原和层粘连蛋白的中断情况，对良性或恶性黑素细胞病变均无特异性，不能用作侵袭性恶性肿瘤的诊断标志物。
