Tan Huan, Thacker Jon, Franklin Tammy, Prasad Pottumarthi V
Department of Surgery (Neurosurgery), University of Chicago, Chicago, Illinois, USA.
J Magn Reson Imaging. 2015 Apr;41(4):1124-8. doi: 10.1002/jmri.24645. Epub 2014 May 6.
To investigate whether arterial spin labeling (ASL) MRI is sensitive to changes by pharmacologically induced vasodilation and vasoconstriction in rat kidneys.
Changes in renal cortical blood flow in seven rats were induced by adenosine infusion (vasodilation) and L-NAME injection (vasoconstriction). All imaging studies were performed on a 3 Tesla scanner using a FAIR-TrueFISP sequence for the ASL implementation. The acquisition time for each ASL scan was 6 min. Cortical perfusion rates were calculated using regions of interest analysis, and the differences in perfusion rates during baseline, vasodilation, and vasoconstriction were compared and assessed for statistical significance.
Compared with the baseline, an average of 94 mL/100 g/min increase and 157 mL/100 g/min decrease in cortical perfusion was observed following adenosine infusion and L-NAME administration, respectively. The changes in cortical perfusion were significant between baseline and vasodilation (P < 0.05), baseline and vasoconstriction (P < 0.01), and vasodilation and vasoconstriction (P < 0.01).
ASL is sensitive to pharmacologically induced perfusion changes in rat kidneys at doses comparable to current use. The preliminary results suggest the feasibility of ASL for investigating renal blood flow in a variety of rodent models.
研究动脉自旋标记（ASL）磁共振成像（MRI）对大鼠肾脏药理学诱导的血管舒张和收缩所引起变化的敏感性。
通过输注腺苷（血管舒张）和注射L-精氨酸甲酯（L-NAME，血管收缩）诱导7只大鼠肾皮质血流发生变化。所有成像研究均在3特斯拉扫描仪上使用用于ASL实施的FAIR-TrueFISP序列进行。每次ASL扫描的采集时间为6分钟。使用感兴趣区分析计算皮质灌注率，并比较和评估基线、血管舒张和血管收缩期间灌注率的差异，以确定其统计学意义。
与基线相比，输注腺苷和给予L-NAME后，皮质灌注分别平均增加94 mL/100 g/min和减少157 mL/100 g/min。皮质灌注在基线与血管舒张之间（P < 0.05）、基线与血管收缩之间（P < 0.01）以及血管舒张与血管收缩之间（P < 0.01）的变化具有显著性。
在与当前使用剂量相当的情况下，ASL对大鼠肾脏药理学诱导的灌注变化敏感。初步结果表明ASL在各种啮齿动物模型中研究肾血流的可行性。
