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集胞藻6803对砷的基因组反应

Genomic responses to arsenic in the cyanobacterium Synechocystis sp. PCC 6803.

作者信息

Sánchez-Riego Ana María, López-Maury Luis, Florencio Francisco Javier

机构信息

Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Sevilla, Spain.

出版信息

PLoS One. 2014 May 5;9(5):e96826. doi: 10.1371/journal.pone.0096826. eCollection 2014.

Abstract

Arsenic is a ubiquitous contaminant and a toxic metalloid which presents two main redox states in nature: arsenite [As(III)] and arsenate [As(V)]. Arsenic resistance in Synechocystis sp. strain PCC 6803 is mediated by the arsBHC operon and two additional arsenate reductases encoded by the arsI1 and arsI2 genes. Here we describe the genome-wide responses to the presence of arsenate and arsenite in wild type and mutants in the arsenic resistance system. Both forms of arsenic produced similar responses in the wild type strain, including induction of several stress related genes and repression of energy generation processes. These responses were transient in the wild type strain but maintained in time in an arsB mutant strain, which lacks the arsenite transporter. In contrast, the responses observed in a strain lacking all arsenate reductases were somewhat different and included lower induction of genes involved in metal homeostasis and Fe-S cluster biogenesis, suggesting that these two processes are targeted by arsenite in the wild type strain. Finally, analysis of the arsR mutant strain revealed that ArsR seems to only control 5 genes in the genome. Furthermore, the arsR mutant strain exhibited hypersentivity to nickel, copper and cadmium and this phenotype was suppressed by mutation in arsB but not in arsC gene suggesting that overexpression of arsB is detrimental in the presence of these metals in the media.

摘要

砷是一种普遍存在的污染物和有毒类金属,在自然界中呈现两种主要的氧化还原状态:亚砷酸盐[As(III)]和砷酸盐[As(V)]。集胞藻PCC 6803菌株中的抗砷性由arsBHC操纵子以及arsI1和arsI2基因编码的另外两种砷酸盐还原酶介导。在此,我们描述了野生型和抗砷系统突变体对砷酸盐和亚砷酸盐存在的全基因组反应。两种形式的砷在野生型菌株中产生了相似的反应,包括诱导几个与应激相关的基因以及抑制能量产生过程。这些反应在野生型菌株中是短暂的,但在缺乏亚砷酸盐转运蛋白的arsB突变体菌株中持续存在。相比之下,在缺乏所有砷酸盐还原酶的菌株中观察到的反应有所不同,包括参与金属稳态和铁硫簇生物合成的基因诱导程度较低,这表明在野生型菌株中这两个过程是亚砷酸盐的作用靶点。最后,对arsR突变体菌株的分析表明,ArsR似乎仅控制基因组中的5个基因。此外,arsR突变体菌株对镍、铜和镉表现出超敏感性,并且这种表型在arsB发生突变时受到抑制,但在arsC基因发生突变时不受抑制,这表明在培养基中存在这些金属的情况下,arsB的过表达是有害的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db85/4010505/90691e029a39/pone.0096826.g001.jpg

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