Dock-Bregeon A C, Chevrier B, Podjarny A, Johnson J, de Bear J S, Gough G R, Gilham P T, Moras D
Institut de Biologie Moléculaire et Cellulaire de C.N.R.S., Strasbourg, France.
J Mol Biol. 1989 Oct 5;209(3):459-74. doi: 10.1016/0022-2836(89)90010-7.
The crystallographic structure of the synthetic oligoribonucleotide, U(UA)6A, has been solved at 2.25 A resolution. The crystallographic refinement permitted the identification of 91 solvent molecules, with a final agreement factor of 13%. The molecule is a dimer of 14 base-pairs and shows the typical features of an A-type helix. However, the presence of two kinks causes a divergence from a straight helix. The observed deformation, which is stabilized by a few hydrogen bonds in the crystal packing, could be due to the relatively high (35 degrees C) temperature of crystallization. The complete analysis of the structure is presented. It includes the stacking geometries, the backbone conformation and the solvation.
合成寡核糖核苷酸U(UA)6A的晶体结构已在2.25埃分辨率下解析出来。晶体学精修确定了91个溶剂分子，最终拟合优度为13%。该分子是一个由14个碱基对组成的二聚体，呈现出典型的A型螺旋特征。然而，两个扭结的存在导致其偏离了直螺旋结构。在晶体堆积中，这种观察到的变形通过一些氢键得以稳定，可能是由于相对较高（35摄氏度）的结晶温度所致。本文给出了该结构的完整分析，包括堆积几何结构、主链构象和溶剂化情况。
