Faria A C S, Schwarz D G G, Carvalho I A, Rocha B B, De Carvalho Castro K N, Silva M R, Moreira M A S
Faculdade de Ciências Biológicas e da Saúde (FACISA/UNIVIÇOSA), Av. Maria de Paula Santana, 3815, Silvestre, CEP 36570-000, Viçosa, Minas Gerais, Brazil.
Departamento de Medicina Veterinária, Universidade Federal de Viçosa (UFV), Av. P. H. Rolfs, s/n, Campus Universitário, CEP 36570-900, Viçosa, Minas Gerais, Brazil.
J Dairy Sci. 2014 Jul;97(7):4111-4. doi: 10.3168/jds.2013-7835. Epub 2014 May 3.
Mycobacterium avium ssp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis and it potentially plays a role in Crohn's disease. In humans, the main route of transmission of MAP might be the intake of contaminated milk and dairy products. Considering that MAP has already been detected in many types of cheese in different counties, and that Coalho cheese is an important dairy product in northeastern Brazil, the aim of this study was to report the first detection of MAP in retail Coalho cheese in Brazil by PCR and culture. Of 30 retail Coalho cheese samples, 3 (10%) amplified fragments of a similar size to that expected (626 bp) were obtained and viable MAP was recovered by culture from 1 (3.3%) sample. The DNA from the positive culture sample was sequenced and showed 99% identity with the insertion sequence IS900 deposited in GenBank. It was possible to identify the presence of MAP-specific DNA in the analyzed samples for the first time in Brazil, and to recover viable cells from retail Coalho cheese.
副结核分枝杆菌（MAP）是副结核病的病原体，它可能在克罗恩病中发挥作用。在人类中，MAP的主要传播途径可能是摄入受污染的牛奶和奶制品。鉴于在不同国家的多种奶酪中已检测到MAP，且科阿略奶酪是巴西东北部的一种重要奶制品，本研究旨在通过聚合酶链反应（PCR）和培养法首次报告在巴西零售的科阿略奶酪中检测到MAP。在30份零售的科阿略奶酪样本中，有3份（10%）扩增出大小与预期（626 bp）相似的片段，并且通过培养从1份（3.3%）样本中分离出了活的MAP。对阳性培养样本的DNA进行测序，结果显示其与GenBank中保存的插入序列IS900有99%的同一性。在巴西首次在分析的样本中鉴定出MAP特异性DNA的存在，并从零售的科阿略奶酪中分离出活细胞。
