Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), 92 Gwahang-no, Yusung-gu, Daejeon, 305-350, Korea,
Bull Environ Contam Toxicol. 2014 Jul;93(1):89-94. doi: 10.1007/s00128-014-1286-6. Epub 2014 May 6.
The biphenyl-degrading strain, Pseudomonas sp. KM-04, was isolated from polychlorinated biphenyls-contaminated soil sample obtained from the vicinity of a former coal mine. We herein report that strain KM-04 can use biphenyl as a sole carbon source, and resting cells convert biphenyl to its corresponding metabolic intermediates. Incubation of KM-04 with autoclaved mining-contaminated soil for 10 days in a slurry system reduced the levels of biphenyl and 2-chlorobiphenyl by 98.5 % and 82.3 %, respectively. Furthermore, treatment of a mine-soil microcosm with strain KM-04 for 15 days in a composting system under laboratory conditions reduced the levels of biphenyl and 2-chlorobiphenyl by 87.1 % and 68.7 %, respectively. These results suggest that KM-04 is a potential candidate for the biological removal of biphenyl and its chlorinated derivatives from polychlorinated biphenyl-contaminated mining areas.
从附近的一个废弃煤矿污染的土壤样本中分离到了能够降解联苯的假单胞菌（Pseudomonas sp.）KM-04 菌株。我们的研究表明，该菌株能够以联苯作为唯一碳源，并且在静息细胞条件下，将联苯转化为相应的代谢中间产物。在泥浆系统中，KM-04 菌株与高温高压灭菌的矿区污染土壤孵育 10 天，分别将联苯和 2-氯联苯的含量降低了 98.5%和 82.3%。此外，在实验室条件下，用 KM-04 菌株处理堆肥系统中的矿区土壤微宇宙 15 天，分别将联苯和 2-氯联苯的含量降低了 87.1%和 68.7%。这些结果表明，KM-04 菌株是一种很有潜力的候选菌株，可以用于从多氯联苯污染的矿区中去除联苯及其氯化衍生物。
