Fava F, Marchetti L
Department of Applied Chemistry and Material Science, Engineering Faculty, University of Bologna, Italy.
Appl Microbiol Biotechnol. 1991 Nov;36(2):240-5. doi: 10.1007/BF00164428.
A mixed bacterial culture obtained from polychlorinated-biphenyl-contaminated river sediments proved capable of degrading 3-chlorobiphenyl (3-CB) under aerobic laboratory conditions. Almost total mineralization of 150 mg/l of 3-CB occurred when, after 3 days of incubation, the mineral medium was supplied with benzoic acid as a carbon source. Two strains of Pseudomonas capable of degrading the substrate to 3-chlorobenzoic acid and a strain of Pseudomonas fluorescens capable of co-metabolizing this metabolite were selected from the mixed culture. A nearly stoichiometric amount of chloride, which defines the percentage of total mineralization, was eliminated during mixed culture growth.
从受多氯联苯污染的河流沉积物中获得的混合细菌培养物，在需氧实验室条件下被证明能够降解3-氯联苯（3-CB）。当在培养3天后向矿物培养基中添加苯甲酸作为碳源时，150 mg/l的3-CB几乎完全矿化。从混合培养物中筛选出了两株能够将底物降解为3-氯苯甲酸的假单胞菌菌株，以及一株能够共代谢这种代谢产物的荧光假单胞菌菌株。在混合培养物生长过程中，消除了几乎化学计量的氯，这确定了总矿化的百分比。
