Department of Biology, Lund University, Lund, Sweden.
Department of Biology, Lund University, Lund, Sweden.
Adv Med Sci. 2014 Mar;59(1):74-80. doi: 10.1016/j.advms.2013.08.004. Epub 2014 Mar 22.
Exocrine pancreatic insufficiency (EPI) induced in young pigs by pancreatic duct ligation (PDL) early after weaning result in total growth deprivation while it has little effect in somewhat older pigs. The main objective was to study effects of EPI on gut structure and function in littermate pigs underwent to PDL at different age.
MATERIAL/METHODS: Pigs, duct-ligated at either 7 (2 weeks post-weaning, PDL-7) or 16 weeks of age (PDL-16), and euthanized at an age of 21-23 weeks together with un-operated littermates were studied. The intestinal in vitro permeability was studied in separate PDL-pigs and compared to un-operated.
Morphometric analysis showed gut mucosal atrophy in the PDL-7 as compared to PDL-16 pigs, while no differences in mucosal disaccharidase activities. The intestinal permeability for different-sized markers was significantly increased in the PDL-pigs compared to the un-operated controls. Analyses of the intestinal digesta showed a total lack of pancreatic enzymes in all PDL-pigs, while instead new, as yet unidentified, enzyme-activities appeared.
All EPI-pigs, independent of age at PDL-operation, displayed adaptive gut changes, however the EPI-pigs operated early after weaning appeared more sensitive, probably related to their gut maturity and possibly explaining the growth arrest seen in these pigs.
在断奶后早期进行胰管结扎（PDL）可导致年轻仔猪发生外分泌胰腺功能不全（EPI），从而导致其完全生长受限，而在年龄稍大的仔猪中则影响较小。主要目的是研究 EPI 对不同年龄进行 PDL 的同窝仔猪肠道结构和功能的影响。
材料/方法：对 7 周龄（断奶后 2 周，PDL-7）或 16 周龄（PDL-16）时进行 PDL 的仔猪进行研究，并与未手术的同窝仔猪一起在 21-23 周龄时处死。分别对 PDL 仔猪进行肠道体外通透性研究，并与未手术仔猪进行比较。
形态计量学分析显示，与 PDL-16 仔猪相比，PDL-7 仔猪的肠道黏膜萎缩，而黏膜二糖酶活性无差异。与未手术对照组相比，PDL 仔猪的肠道通透性对不同大小的标志物均显著增加。对肠道内容物的分析表明，所有 PDL 仔猪均完全缺乏胰腺酶，而出现了新的、尚未鉴定的酶活性。
所有 EPI 仔猪，无论 PDL 手术时的年龄如何，均表现出适应性肠道变化，但在断奶后早期进行 PDL 手术的 EPI 仔猪似乎更敏感，这可能与它们的肠道成熟度有关，可能解释了这些仔猪的生长停滞。
