Lazarou S A, Barbul A, Wasserkrug H L, Efron G
Department of Surgery, Sinai Hospital, Baltimore, Md 21215.
Arch Surg. 1989 Dec;124(12):1429-31. doi: 10.1001/archsurg.1989.01410120079015.
Wound fluid from 10-day-old healing wounds in rats inhibits lymphocyte immune responses. Since severe injury is frequently complicated by immunosuppression as manifested by sepsis, we hypothesized that the wound may be a source of factors that impair host immune responses. Therefore, we studied the effect of systemic wound fluid administration on the survival of rats subjected to an acute peritonitis model. Male Sprague-Dawley rats, fitted with internal jugular catheters 48 hours previously, underwent cecal ligation and puncture with a 23-gauge needle. Immediately after the operation, rats were treated intravenously every 12 hours with either wound fluid obtained from 10-day-old healing wounds and adjusted to 10 mg of protein per milliliter or rat serum. In vitro testing of the wound fluid showed it to be highly inhibitory of thymic lymphocyte mitogenesis. Rats treated with wound fluid had significantly higher mortality after peritonitis than did control rats. The data show that the wound contains factors that can impair host immune responses to sepsis. This suggests that the wound may be the source of posttraumatic host immunosuppression.
来自10日龄大鼠愈合伤口的伤口渗出液会抑制淋巴细胞免疫反应。由于严重损伤常伴有脓毒症所表现出的免疫抑制并发症，我们推测伤口可能是损害宿主免疫反应的因素来源。因此，我们研究了全身给予伤口渗出液对急性腹膜炎模型大鼠存活率的影响。雄性Sprague-Dawley大鼠在48小时前植入颈内导管，然后用23号针头进行盲肠结扎和穿刺。手术后立即每隔12小时给大鼠静脉注射从10日龄愈合伤口获得并调整至每毫升含10毫克蛋白质的伤口渗出液或大鼠血清。伤口渗出液的体外测试表明其对胸腺淋巴细胞有丝分裂具有高度抑制作用。接受伤口渗出液治疗的大鼠在腹膜炎后的死亡率显著高于对照大鼠。数据表明伤口含有可损害宿主对脓毒症免疫反应的因素。这表明伤口可能是创伤后宿主免疫抑制的来源。
