Breslin R J, Barbul A, Kupper T S, Knud-Hansen J P, Wasserkrug H L, Efron G
Department of Surgery, Sinai Hospital, Baltimore, MD 21215.
Arch Surg. 1988 Mar;123(3):305-8. doi: 10.1001/archsurg.1988.01400270039005.
Previously we have noted that fluid obtained from ten-day-old healing wounds noncytotoxically inhibits the blastogenesis of lymphocytes in response to mitogens or antigens. Since these lymphocytic responses are interleukin 2 (IL-2)-mediated, we looked for a specific IL-2 inhibitor in wound fluid. We have found that wound fluid blocks the response of thymic lymphocytes and of two cloned T-helper cell lines (D10 and HT2) to exogenous human recombinant IL-2. The wound fluid enhances fibroblast proliferation, thus demonstrating that its proliferative inhibitory activity is specific for lymphocytes. The findings suggest that wound fluid contains a factor that impairs lymphocyte response to IL-2, probably at the receptor or postreceptor level.
此前我们已经注意到，从愈合10天的伤口中获取的液体可非细胞毒性地抑制淋巴细胞对有丝分裂原或抗原的母细胞化反应。由于这些淋巴细胞反应是由白细胞介素2（IL-2）介导的，我们在伤口液体中寻找一种特异性的IL-2抑制剂。我们发现伤口液体可阻断胸腺淋巴细胞以及两种克隆的T辅助细胞系（D10和HT2）对外源性人重组IL-2的反应。伤口液体可增强成纤维细胞增殖，从而表明其增殖抑制活性对淋巴细胞具有特异性。这些发现提示伤口液体含有一种可能在受体或受体后水平损害淋巴细胞对IL-2反应的因子。
