Sowemimo-Coker S O, Meiselman H J
Department of Physiology and Biophysics, USC School of Medicine, Los Angeles 90033.
Cell Biophys. 1989 Dec;15(3):235-48. doi: 10.1007/BF02989686.
The interaction of cationic anesthetics with biological membranes and the resulting alterations of membrane electrokinetic properties continue to be of current interest. The present study was designed to examine the effects of procaine hydrochloride (PRHCL) on the mobility of human red blood cells (RBC); electrophoretic measurements were made on RBC suspended in phosphate-buffered saline (PBS, pH = 5.0, 7.4, or 9.2), autologous plasma or 3 g% dextran T70/PBS (pH = 7.4), with PRHCL concentrations from 8 x 10(-6) to 8 x 10(-2) M. Low concentrations of PRHCL (8 x 10(-5)-8 x 10(-3) M) significantly (p less than 0.001) increased RBC mobility, with a maximal increase of 8.2% at 8 x 10(-4) M. Conversely, a higher PRHCL concentration (8 x 10(-2) M significantly (p less than 0.001) decreased RBC mobility. Both glutaraldehyde fixation and lipid extraction abolished any PRHCL-induced increase in RBC mobility; the observed increases in mobility for normal cells are, thus, consistent with a mechanism based on expansion of the RBC membrane glycocalyx. Microelectrophoretic methods were also used to study the effect of PRHCL (8 x 10(-4) and 8 x 10(-2) M) on RBC membrane calcium binding, with the results indicating that PRHCL competes with calcium for neuraminate binding sites. We conclude that the observed changes in RBC electrokinetic properties reflect incorporation of PRHCL into the RBC membrane; such changes may be of importance in modulating cell-cell interactions.
阳离子麻醉剂与生物膜的相互作用以及由此引起的膜电动特性的改变一直是当前研究的热点。本研究旨在考察盐酸普鲁卡因（PRHCL）对人红细胞（RBC）迁移率的影响；对悬浮于磷酸盐缓冲盐水（PBS，pH = 5.0、7.4或9.2）、自体血浆或3 g%葡聚糖T70/PBS（pH = 7.4）中的RBC进行电泳测量，PRHCL浓度范围为8×10⁻⁶至8×10⁻² M。低浓度的PRHCL（8×10⁻⁵ - 8×10⁻³ M）显著（p < 0.001）增加RBC迁移率，在8×10⁻⁴ M时最大增加8.2%。相反，较高浓度的PRHCL（8×10⁻² M）显著（p < 0.001）降低RBC迁移率。戊二醛固定和脂质提取均消除了PRHCL诱导的RBC迁移率增加；因此，正常细胞迁移率的增加与基于RBC膜糖萼扩张的机制一致。微电泳方法也用于研究PRHCL（8×10⁻⁴和8×10⁻² M）对RBC膜钙结合的影响，结果表明PRHCL与钙竞争神经氨酸结合位点。我们得出结论，观察到的RBC电动特性变化反映了PRHCL掺入RBC膜中；这种变化可能在调节细胞间相互作用中具有重要意义。
