Bor-Kucukatay M, Yalcin O, Meiselman H J, Baskurt O K
Department of Physiology, Akdeniz University School of Medicine, Antalya, Turkey.
Br J Haematol. 2000 Jul;110(1):82-8. doi: 10.1046/j.1365-2141.2000.02150.x.
The effects of recombinant human erythropoietin (rhEPO) on red blood cell (RBC) rheological properties were investigated in rats. Rats received intramuscular injections of 150 U/kg/d rhEPO for 5 d, following which blood samples were obtained 1, 5 or 10 d later. RBC deformability was assessed by determining cell transit times through 5-microm micropores (CTA) and RBC shape recovery time constants via photometry, aggregation in plasma and dextran was measured by photometry and RBC electrophoretic mobility was determined in a cylindrical electrophoresis system. RBC aggregation was found to be significantly decreased on day 5 after rhEPO treatment (P < 0.05), yet was unchanged from control on days 1 and 10. Mean RBC micropore transit times remained unchanged, but the distributions of transit times were altered; compared with control, the 5th percentiles on both days 1 and 5 were decreased and the 95th percentile on day 1 was elevated. Electrophoretic mobility of RBCs in phosphate-buffered saline was significantly increased on day 5 after rhEPO treatment (P < 0.05), with mobility measurements in dextran 500 (MW = 500 kDa) solutions suggesting that the cells' surface properties related to the formation of a 'depletion layer' may be altered on day 1. These results indicate that the rheological behaviour of RBC as a consequence of rhEPO treatment are temporal and are affected by the presence of reticulocytes as well as by the average age of the circulating cells.
在大鼠中研究了重组人促红细胞生成素（rhEPO）对红细胞（RBC）流变学特性的影响。大鼠接受150 U/kg/d的rhEPO肌肉注射，持续5天，之后在1、5或10天后采集血样。通过测定细胞通过5微米微孔的转运时间（CTA）评估RBC变形性，通过光度法测定RBC形状恢复时间常数，通过光度法测量血浆和右旋糖酐中的聚集情况，并在圆柱形电泳系统中测定RBC电泳迁移率。发现rhEPO治疗后第5天RBC聚集显著降低（P<0.05），但在第1天和第10天与对照组相比无变化。RBC微孔平均转运时间保持不变，但转运时间分布发生改变；与对照组相比，第1天和第5天的第5百分位数均降低，第1天的第95百分位数升高。rhEPO治疗后第5天，RBC在磷酸盐缓冲盐水中的电泳迁移率显著增加（P<0.05），在右旋糖酐500（MW = 500 kDa）溶液中的迁移率测量表明，与“耗尽层”形成相关的细胞表面特性可能在第1天发生改变。这些结果表明，rhEPO治疗导致的RBC流变行为具有时间依赖性，并且受网织红细胞的存在以及循环细胞的平均年龄影响。
