Lin Y J, Edelman J R
Department of Biological Sciences, St John's University, Jamaica, New York 11439.
Cytobios. 1989;58(234-35):135-40.
Sister chromatid exchange (SCE) studies have been performed in a variety of animals, both in vitro and in vivo, as well as in plants. To date, no such studies have been performed in any member of the gerbil sub-family (Gerbillinae). A new sister chromatid differential staining method was used with mice in vivo and has now been applied to the Mongolian gerbil, Meriones unguiculatus. With some modifications, this in vivo method was found to be highly reproducible in gerbils, and had consistently produced many metaphase cells with clear sister chromatid differentiation. The method involves subcutaneous implantation of a 50 mg slow-release BrdU pellet, the fluorochrome Hoechst 33258, phosphate buffering at pH 6.8, and incandescent light exposure.
姐妹染色单体交换（SCE）研究已在多种动物中进行，包括体外和体内实验，也在植物中开展过。迄今为止，尚未在沙鼠亚科（Gerbillinae）的任何成员中进行过此类研究。一种新的姐妹染色单体差异染色方法已在小鼠体内使用，现在已应用于长爪沙鼠（Meriones unguiculatus）。经过一些改进，发现这种体内方法在沙鼠中具有高度可重复性，并且始终能产生许多具有清晰姐妹染色单体分化的中期细胞。该方法包括皮下植入一个50毫克的缓释溴脱氧尿苷（BrdU）药丸、荧光染料Hoechst 33258、pH值为6.8的磷酸盐缓冲液以及白炽灯照射。
