Chen J F, Lin Y J
Cytobios. 1985;44(176):73-87.
Various light sources, including ultraviolet light, mercury, germicidal, fluorescent, and incandescent lamps, were studied for their ability to induce sister chromatid differentiation (SCD) in rat bone marrow cells. The light sources were used along with Hoechst 33258 and Giemsa stains for SCD induction. When those lamps which emit significant amounts of heat were used, 60 degrees C incubation in 2X SSC was found to be unnecessary for SCD induction. A high wattage lamp, a high ambient temperature, a short distance between the lamp and the slides, or a light with 360 nm wavelength, minimized the required exposure time to the light. The pH value of the mounting buffer was also a significant factor. Fluorescent black light and incandescent lamps were found to be ideal light sources for SCD induction.
研究了包括紫外线灯、汞灯、杀菌灯、荧光灯和白炽灯在内的各种光源诱导大鼠骨髓细胞姐妹染色单体分化（SCD）的能力。这些光源与 Hoechst 33258 和吉姆萨染色剂一起用于诱导 SCD。当使用那些会散发大量热量的灯时，发现对于 SCD 诱导而言，在 2X SSC 中 60 摄氏度孵育并非必要。高瓦数的灯、高环境温度、灯与载玻片之间的短距离或波长为 360 纳米的光，可将所需的光照暴露时间减至最短。封片剂缓冲液的 pH 值也是一个重要因素。发现荧光黑光灯和白炽灯是诱导 SCD 的理想光源。
