Renes Johan, Rosenow Anja, Roumans Nadia, Noben Jean-Paul, Mariman Edwin C M
NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
Biochim Biophys Acta. 2014 Sep;1844(9):1511-22. doi: 10.1016/j.bbapap.2014.04.023. Epub 2014 May 5.
Obesity is characterized by dysfunctional white adipose tissue (WAT) that ultimately may lead to metabolic diseases. Calorie restriction (CR) reduces the risk for age and obesity-associated complications. The impact of CR on obesity has been examined with human intervention studies, which showed alterations in circulating adipokines. However, a direct effect of CR on the human adipocyte secretome remains elusive. Therefore, the effect of a 96h low glucose CR on the secretion profile of in vitro cultured mature human SGBS adipocytes was investigated by using proteomics technology. Low-glucose CR decreased the adipocyte triglyceride contents and resulted in an altered secretion profile. Changes in the secretome indicated an improved inflammatory phenotype. In addition, several adipocyte-secreted proteins related to insulin resistance showed a reversed expression after low-glucose CR. Furthermore, 6 novel CR-regulated adipocyte-secreted proteins were identified. Since resveratrol (RSV) mimics CR we compared results from this study with data from our previous RSV study on the SGBS adipocyte secretome. The CR and RSV adipocyte secretomes partly differed from each other, although both treatment strategies lead to secretome changes indicating a less inflammatory phenotype. Furthermore, both treatments induced SIRT1 expression and resulted in a reversed expression of detrimental adipokines associated with metabolic complications.
肥胖的特征是白色脂肪组织（WAT）功能失调，最终可能导致代谢性疾病。热量限制（CR）可降低与年龄和肥胖相关并发症的风险。人类干预研究已经检验了CR对肥胖的影响，这些研究显示循环中的脂肪因子发生了改变。然而，CR对人类脂肪细胞分泌组的直接影响仍然难以捉摸。因此，通过蛋白质组学技术研究了96小时低葡萄糖CR对体外培养的成熟人类SGBS脂肪细胞分泌谱的影响。低葡萄糖CR降低了脂肪细胞甘油三酯含量，并导致分泌谱发生改变。分泌组的变化表明炎症表型有所改善。此外，几种与胰岛素抵抗相关的脂肪细胞分泌蛋白在低葡萄糖CR后表达发生了逆转。此外，还鉴定出6种新的CR调节的脂肪细胞分泌蛋白。由于白藜芦醇（RSV）模拟CR，我们将本研究的结果与我们之前关于SGBS脂肪细胞分泌组的RSV研究数据进行了比较。尽管两种治疗策略都导致分泌组变化，表明炎症表型减轻，但CR和RSV脂肪细胞分泌组部分不同。此外，两种治疗均诱导了SIRT1表达，并导致与代谢并发症相关的有害脂肪因子表达逆转。
