Esteban-Gil A, Grisez C, Prevot F, Florentin S, Decaudin A, Picard-Hagen N, Berthelot X, Ronsin P, Alzieu J P, Marois M, Corboz N, Peglion M, Vilardell C, Liénard E, Bouhsira E, Castillo J A, Franc M, Jacquiet P
Animal Pathology Department, Faculty of Veterinary Sciences, University of Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain.
Parasitol Res. 2014 Jun;113(6):2355-62. doi: 10.1007/s00436-014-3894-9. Epub 2014 May 7.
Bovine besnoitiosis is a chronic and debilitating disease observed in many European countries that may cause important economic losses in cattle. The recent widespread of the parasite in Europe had led the European Food Safety Authority to declare bovine besnoitiosis as a re-emerging disease in Europe. Many aspects of the epidemiology of bovine besnoitiosis such as the main routes of transmission are still unclear and need to be further studied. Among the different hypotheses, a sexual transmission has not yet been investigated. Therefore, the aim of this study was to evaluate the presence of Besnoitia besnoiti DNA in the semen of naturally infected bulls by using a highly sensitive method (real-time qPCR). Both pre-sperm and sperm fractions of 40 bulls, including seronegative (n = 11), seropositive subclinically (n = 17), and seropositive clinically (n = 12) infected animals, were collected by electroejaculation and analyzed by real-time qPCR. No B. besnoiti DNA was detected in 27 pre-sperm and 28 sperm fractions of the 40 examined bulls, suggesting that the transmission of B. besnoiti infection by the semen of chronically infected bulls is very unlikely.
牛贝诺孢子虫病是一种在许多欧洲国家发现的慢性衰弱性疾病，可能给养牛业造成重大经济损失。最近该寄生虫在欧洲广泛传播，导致欧洲食品安全局宣布牛贝诺孢子虫病在欧洲再度出现。牛贝诺孢子虫病流行病学的许多方面，如主要传播途径仍不清楚，需要进一步研究。在不同的假说中，性传播尚未得到研究。因此，本研究的目的是通过使用一种高灵敏度方法（实时定量聚合酶链反应）评估自然感染公牛精液中贝诺孢子虫DNA的存在情况。通过电刺激采精收集了40头公牛的精子前体和精子部分，包括血清阴性（n = 11）、亚临床血清阳性（n = 17）和临床血清阳性（n = 12）的感染动物，并通过实时定量聚合酶链反应进行分析。在40头被检查公牛的27个精子前体和28个精子部分中均未检测到贝诺孢子虫DNA，这表明慢性感染公牛的精液传播贝诺孢子虫感染的可能性非常小。
