Lindberger M, Schröder H D, Schultzberg M, Kristensson K, Persson A, Ostman J, Link H
Department of Pathology, Karolinska Institutet, Huddinge Hospital, Sweden.
J Neurol Sci. 1989 Nov;93(2-3):289-96. doi: 10.1016/0022-510x(89)90198-6.
Standardised skin biopsies followed by immunohistochemical examination for the presence of terminal nerve fibres reacting for neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) were evaluated. Healthy subjects regularly displayed free nerve endings of both fibre types in the papillary and reticular dermis. Both fibre types were present close to blood vessels, while CGRP immunoreactive fibres were more often encountered near sweat gland acini compared to SP fibres. Diabetes mellitus complicated by polyneuropathy was accompanied by marked reduction of SP and CGRP reactive fibres in the dermis layers. Five type I diabetes patients without clinical or neurophysiological evidence of polyneuropathy also had reduced density of both fibre types, being significant for CGRP fibres when compared with controls. Skin biopsy with immunohistochemical staining for neuropeptides may represent a sensitive tool in evaluation of patients with peripheral neuropathies.
对标准化皮肤活检标本进行免疫组织化学检查，以评估是否存在对神经肽P物质（SP）和降钙素基因相关肽（CGRP）产生反应的终末神经纤维。健康受试者在乳头层和网状真皮中经常显示出两种纤维类型的游离神经末梢。两种纤维类型都存在于血管附近，与SP纤维相比，CGRP免疫反应性纤维在汗腺腺泡附近更常见。并发多发性神经病的糖尿病患者真皮层中SP和CGRP反应性纤维明显减少。5例无临床或神经生理学证据表明存在多发性神经病的I型糖尿病患者，两种纤维类型的密度也降低，与对照组相比，CGRP纤维的密度降低具有显著性。对神经肽进行免疫组织化学染色的皮肤活检可能是评估周围神经病患者的一种敏感工具。
