Pastore M, Francioni S
J Nucl Med Allied Sci. 1989 Jul-Sep;33(3 Suppl):107-10.
To-day, the possibility of researching alpha-fetoprotein is given not only by the RIA method but also some immunoenzymatic methods. The Authors have compared three types of methods: RIA, EIA and ELISA, and have also studied the correlation coefficient in 30 samples. Method in solid phase (coated-tubes) and mouse monoclonal antibodies are used for the radioimmunologic method. The ELISA method utilizes two monoclonal antibodies: the former is coated to the walls of the test tubes (mouse monoclonal antibodies) and the latter is labelled with bovine alkaline phosphatase. The EIA methods is a solid phase enzyme immunoassay based on the sandwich principle. It consists of beads coated of goat antibodies and goat anti-AFP antibodies conjugated with horseradish peroxidase. The correlation coefficient is: RIA-ELISA = r 0.95; RIA-EIA = r 0.93; EIA-ELISA = r 0.96. The correlation coefficient from both the RIA method (taken as a reference) and the immunoenzymatic methods is good, so that the passage from RIA to enzymatic methods is possible without problems of under or above dosage of alpha-fetoprotein values.
如今，研究甲胎蛋白的可能性不仅由放射免疫分析方法提供，一些免疫酶法也可实现。作者比较了三种方法：放射免疫分析（RIA）、酶免疫分析（EIA）和酶联免疫吸附测定（ELISA），并研究了30个样本的相关系数。放射免疫法采用固相（包被管）方法和小鼠单克隆抗体。ELISA法使用两种单克隆抗体：前者包被在试管壁上（小鼠单克隆抗体），后者用牛碱性磷酸酶标记。EIA法是基于夹心原理的固相酶免疫测定。它由包被有山羊抗体的珠子和与辣根过氧化物酶偶联的山羊抗甲胎蛋白抗体组成。相关系数为：RIA - ELISA = r 0.95；RIA - EIA = r 0.93；EIA - ELISA = r 0.96。以RIA法（作为参考）和免疫酶法得出的相关系数都很好，因此从RIA法转换为酶法时，甲胎蛋白值的剂量不会出现过低或过高的问题。
