用于甲胎蛋白酶免疫测定的辐射聚合法柔性多孔盘
Flexible porous discs by radiation polymerization method for enzyme immunoassay of alpha-fetoprotein.
作者信息
Kumakura M, Adachi S, Kaetsu I
出版信息
Arch Immunol Ther Exp (Warsz). 1984;32(1):135-41.
New porous discs for enzyme immunoassay of alpha-fetoprotein (AFP) have been prepared by radiation polymerization of various monomers at low temperature. The activity (optical density) of immobilized anti-AFP disc varied with irradiation temperature, anti-AFP concentration, and the hydrophilicity of monomer, in which copolymerization of hydrophilic 2-hydroxyethyl methacrylate and hydrophobic monomers at low temperature below 0 degrees C gave a high activity. The immobilized anti-AFP disc was flexible and had a porous structure. The immobilized anti-AFP discs in dry state are convenient for storage. The minimum serum volume in the assay was 5 microliter. A good correlation (r = 0.98) was noted between AFP concentrations measured by ratio immunoassay and enzyme immunoassay with immobilized anti-AFP disc. The mean recovery of AFP (16-80 ng) added to serum was 95-110%.
通过在低温下对各种单体进行辐射聚合，制备了用于甲胎蛋白（AFP）酶免疫测定的新型多孔圆盘。固定化抗AFP圆盘的活性（光密度）随辐照温度、抗AFP浓度和单体亲水性而变化，其中在低于0℃的低温下，亲水性甲基丙烯酸2-羟乙酯与疏水性单体共聚可产生高活性。固定化抗AFP圆盘具有柔韧性且呈多孔结构。干燥状态下的固定化抗AFP圆盘便于储存。该测定中血清的最小体积为5微升。用固定化抗AFP圆盘进行的比率免疫测定和酶免疫测定所测得的AFP浓度之间具有良好的相关性（r = 0.98）。添加到血清中的AFP（16 - 80纳克）的平均回收率为95 - 110%。
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