Thu Nguyen Binh Anh, Nguyen Quang Thien, Hoang Xuan Lan Thi, Thao Nguyen Phuong, Tran Lam-Son Phan
School of Biotechnology, International University, Vietnam National University HCMC, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 70000, Vietnam.
Signaling Pathway Research Unit, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
Biomed Res Int. 2014;2014:809736. doi: 10.1155/2014/809736. Epub 2014 Apr 7.
Drought is one of the greatest constraints to soybean production in many countries, including Vietnam. Although a wide variety of the newly produced cultivars have been produced recently in Vietnam through classical breeding to cope with water shortage, little knowledge of their molecular and physiological responses to drought has been discovered. This study was conducted to quickly evaluate drought tolerance of thirteen local soybean cultivars for selection of the best drought-tolerant cultivars for further field test. Differences in drought tolerance of cultivars were assessed by root and shoot lengths, relative water content, and drought-tolerant index under both normal and drought conditions. Our data demonstrated that DT51 is the strongest drought-tolerant genotype among all the tested cultivars, while the highest drought-sensitive phenotype was observed with MTD720. Thus, DT51 could be subjected to further yield tests in the field prior to suggesting it for use in production. Due to their contrasting drought-tolerant phenotypes, DT51 and MTD720 provide excellent genetic resources for further studies underlying mechanisms regulating drought responses and gene discovery. Our results provide vital information to support the effort of molecular breeding and genetic engineering to improve drought tolerance of soybean.
干旱是包括越南在内的许多国家大豆生产面临的最大制约因素之一。尽管越南最近通过传统育种培育出了各种各样的新品种以应对缺水问题,但对它们在干旱条件下的分子和生理反应了解甚少。本研究旨在快速评估13个本地大豆品种的耐旱性,以筛选出最佳耐旱品种用于进一步的田间试验。通过在正常和干旱条件下测定品种的根长、茎长、相对含水量和耐旱指数来评估品种间耐旱性差异。我们的数据表明DT51是所有测试品种中耐旱性最强的基因型,而MTD720表现出最高的干旱敏感表型。因此,在建议DT51用于生产之前,可对其进行进一步田间产量测试。由于DT51和MTD720具有截然不同的耐旱表型,它们为进一步研究调控干旱反应的机制和基因发现提供了优良的遗传资源。我们的结果为支持大豆耐旱性分子育种和基因工程研究提供了重要信息。