Salonen J I, Kautsky M B, Dale B A
J Periodontal Res. 1989 Nov;24(6):370-7. doi: 10.1111/j.1600-0765.1989.tb00885.x.
The relationship between cell attachment and the phenotype of the attached oral epithelial cells was studied by comparing junctional epithelium (JE) with a culture model for JE in which epithelial cells form an equivalent organization of tissues. Gingival explants were cultured on either a high or a low protein-binding membrane. The cut edge of epithelium and connective tissue was placed on the membrane; epithelial cells migrated to form a sheet of tissue between the explant and the membrane substratum. Cells which grew in contact with the high protein-binding membrane attached to the substratum and assumed a cuboidal shape. With time in culture these cells showed a decrease in reactivity with antibodies to psi-3 antigen (an antigen associated with epithelial migration) and an increase in reactivity with antibodies to cytokeratin 19 (a marker for JE). Cells grown on the low protein-binding membrane did not exhibit changes in shape or antigens. Because similar features were found in the JE in vivo, it was concluded that the junctional cells which are directly attached to the tooth (DAT cells) have a nonmigratory phenotype that develops as a response to the tooth surface. Because the cells are in contact with a metabolically inert material the changes appear to be largely self-induced. The culture method allows studies on putative inducer molecules and on mechanisms which may control the phenotype of epithelial cells at the dentogingival interface.
通过将结合上皮（JE）与JE的培养模型进行比较，研究了细胞附着与附着的口腔上皮细胞表型之间的关系，在该培养模型中上皮细胞形成了等效的组织结构。牙龈外植体在高蛋白结合膜或低蛋白结合膜上培养。上皮和结缔组织的切缘置于膜上；上皮细胞迁移，在植体和膜基质之间形成一层组织。与高蛋白结合膜接触生长的细胞附着于基质并呈立方形。随着培养时间的延长，这些细胞与psi-3抗原（一种与上皮迁移相关的抗原）抗体的反应性降低，与细胞角蛋白19（JE的标志物）抗体的反应性增加。在低蛋白结合膜上生长的细胞在形状或抗原方面未表现出变化。由于在体内JE中发现了类似特征，得出的结论是，直接附着于牙齿的结合细胞（DAT细胞）具有非迁移表型，该表型是对牙齿表面的反应而形成的。由于这些细胞与代谢惰性物质接触，这些变化似乎很大程度上是自我诱导的。该培养方法允许对假定的诱导分子以及可能控制牙-龈界面上皮细胞表型的机制进行研究。
