通过超高效液相色谱/电喷雾电离-四极杆飞行时间质谱法快速鉴定RAW264.7细胞中的甘油磷脂。
Rapid identification of glycerophospholipids from RAW264.7 cells by UPLC/ESI -QTOF-MS.
作者信息
She Yuqi, Song Jianao, Yang E, Zhao Lili, Zhong Yanmei, Rui Wen, Feng Yifan, Wu Xia
机构信息
Central Laboratory, Guangdong Pharmaceutical University, Guangzhou, 510006, People's Republic of China.
出版信息
Biomed Chromatogr. 2014 Dec;28(12):1744-55. doi: 10.1002/bmc.3216. Epub 2014 May 8.
The study aims to develop a rapid, sensitive ultra-performance liquid chromatography coupled with an electrospray ionization quadruple time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) analytical method for identifying glycerophospholipids (GPLs) from RAW264.7 cells. A total of 78 GPLs including 22 phosphatidylethanolamines (PEs), 49 phosphatidylcholines (PCs), four phosphatidylglycerols, one phosphatidylinositol and two unknown GPLs were identified. PC (14:0/16:1), PC (14:0/16:0), PE (0:0/20:3), PE (22:5/0:0) and PE (22:3/0:0) were identified for the first time. The UPLC/Q-TOF-MS method is suitable for targeting analysis of GPLs from RAW264.7 cells, which allows us to find out new GPLs compositions related to inflammatory diseases and to explain their pharmacological roles in inflammatory process.
本研究旨在开发一种快速、灵敏的超高效液相色谱-电喷雾电离四极杆飞行时间串联质谱联用（UPLC/Q-TOF-MS）分析方法，用于鉴定RAW264.7细胞中的甘油磷脂（GPLs）。共鉴定出78种GPLs，包括22种磷脂酰乙醇胺（PEs）、49种磷脂酰胆碱（PCs）、4种磷脂酰甘油、1种磷脂酰肌醇和2种未知的GPLs。首次鉴定出PC（14:0/16:1）、PC（14:0/16:0）、PE（0:0/20:3）、PE（22:5/0:0）和PE（22:3/0:0）。UPLC/Q-TOF-MS方法适用于RAW264.7细胞中GPLs的靶向分析，这使我们能够找出与炎症性疾病相关的新GPLs组成，并解释它们在炎症过程中的药理作用。
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