Hu Baoji, Zou Yun, Liu Shanshan, Wang Jun, Zhu Jiali, Li Jinbao, Bo Lulong, Deng Xiaoming
Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China ; Department of Anesthesiology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gongwei Road, Huinan Town, Pudong, Shanghai 201399, China.
Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
Mediators Inflamm. 2014;2014:314081. doi: 10.1155/2014/314081. Epub 2014 Apr 7.
Salidroside, isolated from the medicinal plant Rhodiola, was reported to serve as an "adaptogen." This study was designed to explore the protective effect of salidroside on concanavalin A- (Con A-) induced hepatitis in mice and investigate potential mechanisms. C57BL/6 mice were randomly divided into control group, Con A group, and salidroside group. Salidroside (50 mg/kg) was injected intravenously followed by Con A administration. The levels of ALT, AST, inflammatory cytokines and CXCL-10 were examined. The pathological damage of livers was assessed, the amounts of phosphorylated IκBα and p65 were measured, and the numbers of CD4(+) and CD8(+) T lymphocytes in the blood, spleen and infiltrated in the liver were calculated. Our results showed that salidroside pretreatment reduced the levels of ALT, AST dramatically and suppressed the secretion of proinflammatory cytokines through downregulating the activity of NF-κB partly. Salidroside altered the distribution of CD4(+) and CD8(+) T lymphocyte in the liver and spleen through regulating CXCL-10 and decreased the severity of liver injuries. In conclusion, these results confirm the efficacy of salidroside in the prevention of immune mediated hepatitis in mice.
红景天苷是从药用植物红景天中分离得到的，据报道它具有“适应原”的作用。本研究旨在探讨红景天苷对刀豆蛋白A（Con A）诱导的小鼠肝炎的保护作用，并研究其潜在机制。将C57BL/6小鼠随机分为对照组、Con A组和红景天苷组。静脉注射红景天苷（50mg/kg）后给予Con A。检测谷丙转氨酶（ALT）、谷草转氨酶（AST）、炎性细胞因子和CXCL-10的水平。评估肝脏的病理损伤，检测磷酸化IκBα和p65的含量，并计算血液、脾脏及肝脏浸润的CD4(+)和CD8(+) T淋巴细胞数量。我们的结果表明，红景天苷预处理可显著降低ALT、AST水平，并通过部分下调NF-κB活性抑制促炎细胞因子的分泌。红景天苷通过调节CXCL-10改变肝脏和脾脏中CD4(+)和CD8(+) T淋巴细胞的分布，并减轻肝脏损伤的严重程度。总之，这些结果证实了红景天苷在预防小鼠免疫介导性肝炎方面的有效性。
