玉米（Zea mays）种子中不依赖2,3-二磷酸甘油酸的磷酸甘油酸变位酶的纯化、特性及免疫学性质

Purification, characterization and immunological properties of 2,3-bisphosphoglycerate-independent phosphoglycerate mutase from maize (Zea mays) seeds.

作者信息

Graña X, Ureña J, Ludevid D, Carreras J, Climent F

机构信息

Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.

出版信息

Eur J Biochem. 1989 Dec 8;186(1-2):149-53. doi: 10.1111/j.1432-1033.1989.tb15189.x.

DOI:10.1111/j.1432-1033.1989.tb15189.x

Abstract

2,3-Bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) was purified and characterized from maize. SDS electrophoresis showed only one band with a molecular mass of 64 kDa, similar to that determined for the native enzyme by gel-filtration chromatography. The kinetic constants were similar to those reported for wheat germ phosphoglycerate mutase. Rabbit antiserum against maize phosphoglycerate mutase possesses a high degree of specificity. It also reacts with the wheat germ enzyme but fails to react with other cofactor-independent or cofactor-dependent phosphoglycerate mutases. Cell-free synthesis experiments indicate that phosphoglycerate mutase from maize is not post-translationally modified.

摘要

从玉米中纯化并鉴定了不依赖2,3-二磷酸甘油酸的磷酸甘油酸变位酶（EC 5.4.2.1）。SDS电泳显示只有一条分子量为64 kDa的条带，这与通过凝胶过滤色谱法测定的天然酶的分子量相似。动力学常数与报道的小麦胚芽磷酸甘油酸变位酶的动力学常数相似。针对玉米磷酸甘油酸变位酶的兔抗血清具有高度特异性。它也与小麦胚芽酶发生反应，但不与其他不依赖辅因子或依赖辅因子的磷酸甘油酸变位酶发生反应。无细胞合成实验表明，玉米中的磷酸甘油酸变位酶没有进行翻译后修饰。

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